10种提取液对菊花花瓣中类胡萝卜素提取效率的影响  

Analysis on Efficiency of Ten Extracts for Carotenoid Content in Petals of Chrysanthemum

作　　者：车越[1] 王普[1] 孙卫[1] 戴思兰[1] 

机构地区：[1]北京林业大学/国家花卉工程技术研究中心,北京100083

出　　处：《湖北农业科学》2011年第15期3152-3155,共4页Hubei Agricultural Sciences

基　　金：国家大学生创新性实验计划(GCS08038);国家863计划项目(2006AA100109);国家林业局林业行业公益性科研专项(200904050)

摘　　要：类胡萝卜素是菊花中一类重要的呈色物质,高效提取类胡萝卜素对于分离和鉴定色素组成以及研究菊花的呈色机理十分重要。分别采用丙酮、甲醇、无水乙醇、无水乙醚、石油醚、丙酮:石油醚不同体积比(1:1、1:2、1:4、2:1、4:1)10种提取液,提取菊花品种光辉及ReaganOrange舌状花中的的类胡萝卜素,用紫外-可见光分光光度计测定提取液中类胡萝卜素的相对含量。结果发现,不同提取液中菊花类胡萝卜素的提取量不同;醇类为菊花中类胡萝卜素较适合的提取液。结果还表明,菊花中含氧类胡萝卜素含量较高。Carotenoid is an important kind of coloring material in chrysanthemum,and extracting this component efficiently is very important for the isolation and identification of pigment composition and the coloring mechanism of chrysanthemum.10 different extraction solutions were used,including petroleum ether,methanol,anhydrous ethyl alcohol,anhydrous ether,acetone,acetone：petroleum ether in different ratios （1：1;1：2;1：4;2：1;4：1） to extract carotenoids in the petals of chrysanthemum ＇Brilliant＇ and ＇Reagan Orange＇.The relative content of total carotenoids was calculated from UV-VIS spectrometer.The results showed that the quantity of extracts varied in 10 extracts of chrysanthemum;Alcohol was more suitable for extracting the carotenoids of chrysanthemum;It also indicated that most of the carotenoids in chrysanthemum was oxygenated compounds and it could lay the foundation for the study of compounds and content of carotenoid in chrysanthemum.

关 键 词：菊花花瓣 类胡萝卜素 提取液 

分 类 号：S682.11[农业科学—观赏园艺]