济宁青山羊发情周期不同阶段LHR在输卵管的分布及其mRNA表达  

Localization and Differential Expression of LHR and LHR mRNA in the Oviduct of Jining Gray Goat During Estrous Cycle

作　　者：李广君[1,2] 王慧[1,2] 王树迎[1,2] 侯衍猛[1,2] 黄丽波[1,2] 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018 [2]山东农业大学动物科技学院/动物生物技术与疫病防治重点实验室,山东泰安271018

出　　处：《中国农业科学》2011年第15期3235-3245,共11页Scientia Agricultura Sinica

基　　金：国家自然科学基金项目(30871832);山东省科技攻关项目(2006GG2209004)

摘　　要：【目的】探究济宁青山羊发情周期不同阶段黄体生成素受体(LHR)在输卵管不同部位的分布及其mRNA的表达差异。【方法】运用实时荧光定量RT-PCR技术,对LHR mRNA目的片段回收、克隆及序列分析,检测其表达量的差异,同时做LHR免疫组化染色。【结果】LHR阳性物质存在于发情周期各个阶段的输卵管各段,且主要分布在输卵管的黏膜上皮细胞、固有层细胞以及血管内皮细胞中。LHR mRNA在整个发情周期的输卵管中均有表达,但以输卵管漏斗部在发情期的相对表达量最高,且与其它3个阶段差异极显著(P<0.01)。输卵管壶腹部在发情后期的相对表达量最低,与其它3个时期的差异显著(P<0.05)。输卵管峡部在发情前期相对表达量最高,与其它3个时期的差异极显著(P<0.01)。【结论】LHR及其mRNA在山羊输卵管分布及表达反映LH对输卵管功能具有一定调节作用。[Objective] The objective of the experiment is to study the localization of LH receptor （LHR） and the expression of LHR mRNA in the oviduct during estrous cycle in Jining Gray Goat. [Method] The target fragment of LHR mRNA was retrieved, cloned and analyzed by using the real time fluorescence quantitative PCR, then the differences in expression were detected. At the same time, the immunohistochemical staining of LHR was done. [Result] The localizations of LHR immunoreactive substance in all parts of the oviduct at different stages of estrous cycle were carried out respectively, and it was found that LHR is mainly distributed on the epithelium of tubal mucosa, lamina propria and blood vessel endothelium. LHR mRNA expression was different in the oviduct of Jining Gray Goat at the four estrous cycle phrases. LHR mRNA in the linfundibulum was the highest during estrus （P〈0.01）. In the ampolla, was the lowest during metestrus （P〈0.05）. In the isthmus, was the highest during proestrus （P〈0.01）. [ Conclusion] The localization of LHR and its mRNA expression in the oviduct of goat reveals that LH has a kind of administration on the function of ovidct.

关 键 词：济宁青山羊 黄体生成素受体 荧光定量RT—PCR 免疫组化 输卵管 

分 类 号：S827[农业科学—畜牧学]