不同主产地黑顺片的指纹图谱研究  

Studies on HPLC Fingerprint of Heishunpian from Different Origin

作　　者：黄慧莲[1] 刘科兰[1] 邵峰[1] 任刚[1] 段锋[1] 刘荣华[1] 

机构地区：[1]江西中医学院现代中药制剂教育部重点实验室,南昌330004

出　　处：《中国实验方剂学杂志》2011年第16期44-49,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30801520);国家中医药管理局中医药行业科研专项(200807020)

摘　　要：目的:研究四川江油、陕西汉中及云南丽江等附子主产区黑顺片的高效液相色谱指纹图谱,为建立附子的行业质量标准提供依据。方法:利用HPLC方法,色谱条件Alltimate C18色谱柱(4.6 mm×250 mm,5μm),柱温35℃,流动相40mmol.L-1醋酸铵(氨水调pH 10.00)-乙腈梯度洗脱,流速1.0 mL.min-1,检测波长为240 nm。对不同主产地的22批黑顺片进行研究,采用药典委员会颁布的中药色谱指纹图谱相似度评价系统2004A和聚类分析对指纹图谱进行了分析。结果:22批黑顺片有11个共有峰,江油地区(道地产区)的黑顺片之间的相似度均大于0.900,非道地产区与道地产区的相似度小于0.900。结论:该方法精密度、重复性和稳定性良好,可结合黑顺片含量测定用于全面控制附子炮制品(黑顺片)的质量。Objective： To study the fingerprints of Heishunpian from Sichuan Jiangyou,Shanxi Hanzhong and Yunnan Lijiang by high performance liquid chromatographic and establish the method of standard quality for Radix Aconiti Lateralis Praeparata.Method： HPLC method was used to establish the fingerprints of 22 batches of Heishunpian.Alltimate C18 column（4.6 mm×250 mm,5 μm） was used,and ammonium acetate solution-acetonitrile as the mobile phase,with a flow rate of 1.0 mL·min-1 and a detection wavelength at 240 nm.The obtained data was analyzed by computer aided similarity evaluation and cluster analysis software.Result： There were 11 common peaks in 22 habitats of Heishunpian.The similarity degree of the products produced in Jiangyou was found to be higher than 0.900.Conclusion： The method is accurate,repeatable and reliable,which can be used for their quality control.

关 键 词：黑顺片 高效液相 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]