RP-HPLC同时测定宣肺止咳口服液中盐酸麻黄碱、盐酸伪麻黄碱及苦杏仁苷含量  

Simultaneous Determination of Ephedrine Hydrochloride,D-pseudo-ephedrine and Amygdalin in Xuanfei Zhike Oral Liquid by RP-HPLC

作　　者：刘睿[1] 王宁[2] 刘志辉[1] 

机构地区：[1]南京中医药大学附属医院,南京210029 [2]南京中医药大学,南京210046

出　　处：《中国实验方剂学杂志》2011年第16期91-93,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:建立同时测定宣肺止咳口服液中盐酸麻黄碱、盐酸伪麻黄碱、苦杏仁苷含量的高效液相色谱测定方法。方法:采用Hedera ODS-2色谱柱(4.6 mm×250 mm,5μm);以乙腈-0.2%磷酸水溶液为流动相梯度洗脱;紫外检测波长210nm,0～24 min流速为1.0 mL.min-1,用于检测盐酸麻黄碱和盐酸伪麻黄碱;27～55 min流速为0.7 mL.min-1,用于检测苦杏仁苷,柱温25℃。结果:盐酸伪麻黄碱、盐酸麻黄碱、苦杏仁苷分别在0.043 2～0.684μg(r=0.999 9),0.048 8～0.976μg(r=0.999 9),0.047 0～0.940 8μg(r=0.999 8)线性关系良好;平均回收率(n=6)分别为96.15%,96.09%,95.74%,RSD分别为0.78%,1.18%,1.80%。结论:该法可以用于宣肺止咳口服液中盐酸麻黄碱、盐酸伪麻黄碱、苦杏仁苷的含量测定。Objective： To establish an HPLC method for determination of ephedrine hydrochloride,D-pseudo-ephedrine and amygdalin in Xuanfei Zhike Oral Liquid.Method： The separation was performed on an Hedera ODS-2（4.6 mm×250 mm,5 μm）;the mobile phase consisted of acetonitrile-0.2%phosphoric acid（5：95） with the flow rate of 1 mL·min-1 for the determination of ephedrine hydrochloride,D-pseudo-ephedrine and 0.7 mL·min-1 for amygdalin,UV detection wavelength was at 210 nm and column temperature was 25 ℃.Result： ephedrine hydrochloride,D-pseudo-ephedrine and amygdalin were linear in the range of 0.043 2-0.684 μg（r=0.999 9）,0.048 8-0.976 μg（r=0.999 9） and 0.047 0-0.940 8 μg（r=0.999 8）,the average recoveries（n=6） of ephedrine hydrochloride,D-pseudo-ephedrine and amygdalin were 96.15%,96.09% and 95.74% with RSD of 0.78%,1.18% and 1.80% respectively.Conclusion： This method is rapid and accurate for the simultaneous analysis of ephedrine hydrochloride,D-pseudo-ephedrine and amygdalin and can provide a reliable way for the quality control for Xuanfei Zhike Oral Liquid.

关 键 词：高效液相色谱 宣肺止咳口服液 盐酸麻黄碱 盐酸伪麻黄碱 苦杏仁苷 

分 类 号：R284.1[医药卫生—中药学]