氧化苦参碱微球冻干针剂器官靶向性研究  

Targeting Investigation for Freeze-dried Injection of Oxymatrine-Polybutylcyanoacrylate-Nanoparticles

作　　者：倪晨[1] 赖春丽[2] 王倩[1] 熊艺花[1] 赖小平[1] 

机构地区：[1]广州中医药大学,广州510006 [2]广州医学院附属肿瘤医院,广州510000

出　　处：《中国实验方剂学杂志》2011年第16期203-206,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:研究氧化苦参碱微球(OMAT-PBCA-NP)冻干针剂的动物器官靶向性。方法:采用高效液相色谱法测定给药大鼠各主要脏器的氧化苦参碱含量。色谱条件:Kromasil C18柱(4.60 mm×250 mm,5μm),流动相甲醇-水-三乙胺(50∶50∶0.02),柱温20℃,检测波长210 nm,流速1 mL.min-1,进样量为10μL。结果:氧化苦参碱在50～400 mg.L-1线性关系良好(r=0.999 0),在30 min,1,4,8 h等时间点中,氧化苦参碱在肝内的含量最高,与其他脏器含量有极显著性差异(P<0.01)。结论OMAT-PBCA-NP具有良好的肝脏靶向性。Objective： To study the organ targeting for freeze-dried injection of Oxymatrine-Polybutylcyanoacrylate-Nanoparticles（OMAT-PBCA-NP） in rats.Method：The content of oxymatrine in major organs of rats after injected with OMAT-PBCA-NP was investigated by high performance liquid chromatography.HPLC was carried out on a Kromasil C18 column（4.60 mm×250 mm,5 μm）with the mobile phase consisting of methanol and water and triethylamine（50∶50∶0.02）,the column temperature was 20 ℃ and UV detection wavelength was 210 nm,the flow was 1 mL·min-1 and the injection volume was 10 μL.Result：The calibration curve of oxymatrine was linear in the concentration range of 50-400 mg·L-1（r=0.999 0）,in 30 min,1,4,8 h,the content of oxymatrine was highest in the liver with significant difference from other organs（P0.01）.Conclusion： OMAT-PBCA-NP has good targeting for liver.

关 键 词：氧化苦参碱微球 靶向性 HPLC 

分 类 号：R285.5[医药卫生—中药学]