大黄对LPS损伤肺泡Ⅱ型上皮细胞水通道蛋白AQP_1及AQP_5 mRNA表达的影响  

Effect of Rhei Radix et Rhigoma on AQP_(1,5) mRNA Expression of Alveolar Type Ⅱ Cell Injured by LPS

作　　者：巫莉萍[1] 邓时贵 黄海定[1] 

机构地区：[1]江西省赣州市人民医院药剂科,江西赣州341000 [2]广州中医药大学第二附属医院 [3]广东省中医药科学院动物实验中心,广州510006

出　　处：《中国实验方剂学杂志》2011年第16期213-217,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广东省科技厅(83028;2009B060300025);广东省中医药管理局(2008318)广州中医药大学(08CX82);赣州市医办字[2010]53号-5)

摘　　要：目的:探讨大黄对脂多糖(Lip polysaccharides,LPS)损伤肺泡Ⅱ型上皮细胞(alveolar typeⅡ,ATⅡ)水通道蛋白(AQP1,AQP5)mRNA表达的影响。方法:原代分离提取纯化大鼠ATⅡ后,分5组:正常对照组加入2 mL DMEM培养基,LPS细胞模型组加10μg.mL-1 LPS致ATⅡ损伤模型,大黄含药血清3组(每组分别再加入占总体积5%,10%,20%的大黄含药血清)。4 h后收集细胞,用RT-PCR方法检测水通道蛋白AQP1,AQP5 mRNA表达。结果:与正常组比较,LPS模型组AQP1mRNA表达显著降低(P<0.05);含20%大黄血清可显著提高AQP1 mRNA表达(P<0.05),但未能恢复至正常水平。与正常组比较,LPS模型组AQP5 mRNA表达升高,但无显著差异;大黄各干预组对AQP5 mRNA高表达有抑制作用,但抑制作用不明显。结论:大黄对急性肺损伤的保护机制可能与上调AQP1 mRNA、下调AQP5 mRNA的表达有关。Objective： To observe effect of Rhei Radix et Rhigoma on AQP1,5 mRNA expression of alveolar type Ⅱcell（ATⅡ） injured by LPS.Method： Primary cultured alveolar type Ⅱcells were divided into 5 groups： control group： 2mL DMEM cultured medium was added;LPS injured cell model group（acute lung injury cell model group）： 10 μg·mL-1 LPS was added to injure AT Ⅱ cell;serum containing Rhei Radix et Rhigoma intervention group was divided into 3 groups： each group was added the total volume of 5%,10%,20% serum containing Rhei Radix et Rhigoma.Which were collected After 4 hours of culture the mRNA expression of AQP1,AQP5 was detected by Real-Time PCR.Result： Compared with the control group,AQP1 mRNA expression of LPS injured cell model group was significantly lower（P0.05）.Compared with the LPS group,20% of total volume of serum containing Rhei Radix et Rhigoma intervention group could increase AQP1 mRNA expression significantly,but not return to the normal level.Compared with the control group,AQP5 mRNA expression of LPS injured cell model group was increased.Rhei Radix et Rhigoma intervention groups could decrease AQP5 mRNA expression,but not significantly.Conclusion： The protective mechanism of Rhei Radix et Rhigoma on acute lung injury cell model by LPS may be associated with the increased AQP1 mRNA expression and the decreased AQP5 mRNA expression.

关 键 词：急性肺损伤 肺泡Ⅱ型上皮细胞 大黄 水通道蛋白-1 水通道蛋白-2 

分 类 号：R285.5[医药卫生—中药学]