几种血管活性药物对失血性休克大鼠肠系膜微循环的影响  

Effects of Several Vasoactive Agent on Intestines Mesentery Microcirculation in Haemorrhagic Shock Rat

作　　者：余上斌[1] 杨莹[1] 柯丹[1] 冯秀玲[1] 晏汉姣[1] 

机构地区：[1]华中科技大学同济医学院机能学中心实验室,武汉430030

出　　处：《微循环学杂志》2011年第3期19-20,25,I0001,共4页Chinese Journal of Microcirculation

摘　　要：目的:比较几种血管活性药物对失血性休克大鼠肠系膜微循环的影响。方法:选取SD大鼠,随机分为正常对照组(A组),失血性休克模型组(B组),生理盐水处理组(C组),多巴胺处理组(D组),去甲肾上腺素处理组(E组),山莨菪碱处理组(F组)。实验采用动脉放血至动脉血压为60mmHg左右,同时观察肠系膜微循环状态改变来复制失血性休克大鼠模型;各组行相应药物处理后,分别测量动脉血压和检测肠系膜微循环相关指标,并进行比较分析。结果:B组大鼠动脉血压、肠系膜微血管出/入口管径、血流速度明显低于A组(P<0.05或P<0.01),血液流态由线流改变为粒流。与B组比较,几种血管活性药物(D、E、F组)对失血性休克大鼠动脉血压均有显著升高作用,依次为去甲肾上腺素>多巴胺>山莨菪碱(P<0.05或P<0.01),多巴胺对微血管出/入口管径恢复明显(P<0.01),山莨菪碱和多巴胺对肠系膜血流速度有明显改善(P<0.01或P<0.05),山莨菪碱还能使肠系膜血流流态恢复为线流。结论:实验性治疗失血性休克需适度补充血容量,同时选用山莨菪碱改善肠系膜微循环,有利于休克复苏。Objective: To investigate the effect of three vasoactive drugs on mesenteric microcirculation during hemorrhagic shock.Method: Male Sprague-Dawley rats were divided into 6 groups which were control group(group A),hemorrhagic shock group(group B),hemorrhagic shock group with normal saline treatment(group C),hemorrhagic shock group with dopamine treatment(group D),hemorrhagic shock group with noradrenalin treatment(group E),hemorrhagic shock group with anisodamine treatment(group F).Method of bloodletting to decrease the BP to the level of 60mmHg was used to establish the animal model of hemorrhagic shock,and recorded parameters to evaluate the effect of different types of drugs on hemorrhagic shock.Results: Hemorrhagic shock decreased the BP,calibres of both inlet and oulet of the mesenteric microcirculation,and the velocity of blood flow.Treatment of normal saline during hemorrhagic shock could only increase BP back to the normal level without improving the mesenteric microcirculation.Dopamine treatment deteriorate the condition by attenuating BP and blood velosity,meanwhile expand the microcirculation(P<0.05).Compare with group A,noradrenalin treatment could partly reverse the impairment of BP and mesenteric microcirculation during hemorrhagic shock(P<0.05~0.01).Interestingly,only could anisodamine rescue the parameters to the normal level.Conclusion: Anisodamine with transfusion treatment could improve mesenteric microcirculation during hemorrhagic shock.These data suggest that anisodamine could serve as a potential therapeutic agent for preventing shock pathological changes via modulating mesenteric microcirculation.

关 键 词：血管活性药物 失血性休克 微循环 大鼠 

分 类 号：R657.2[医药卫生—外科学] R331.35[医药卫生—临床医学]