SEPT4蛋白在特发性弱精子症患者精子中的表达  

Expression of SEPT4 protein in the ejaculated sperm of idiopathic asthenozoospermic men

作　　者：李玉山[1] 冯晓霞[1] 吉晓菲[1] 王全先[1] 高学敏[1] 杨险峰[1] 潘周辉[1] 孙琳[1] 马奎[1] 

机构地区：[1]郑州大学第三附属医院人类精子库,河南郑州450052

出　　处：《中华男科学杂志》2011年第8期699-702,共4页National Journal of Andrology

基　　金：郑州市科技攻关计划项目(112PPTSF318-7)~~

摘　　要：目的:探讨SEPT4蛋白在特发性弱精子症发生机制中的作用。方法:采用非连续密度梯度离心分离和纯化精子,免疫细胞化学技术检测SEPT4在特发性弱精子症患者和正常男性精子中的定位及表达变化,同时用RT-PCR和W estern印迹技术,从基因和蛋白水平检测SEPT4的表达及差异。结果:免疫细胞化学表明,SEPT4定位于精子环并且在特发性弱精子症患者精子中的表达显著低于正常男性(94.75±19.95vs111.51±17.57,t=3.452,P<0.01);RT-PCR显示,特发性弱精子症患者精子中SEPT4 mRNA的表达水平与正常男性相比显著降低(0.56±0.15vs0.71±0.16,t=3.521,P<0.05);W estern印迹结果与RT-PCR结果一致,SEPT4蛋白在特发性弱精子症患者精子中的表达亦显著低于正常男性(0.48±0.20vs0.77±0.18,t=5.872,P<0.05)。结论:SEPT4在特发性弱精子症患者精子中的表达水平显著降低,可能是导致弱精子症发生的原因之一。Objective： To investigate the role of the SEPT4 protein in the pathogenesis of idiopathic asthenozoospermia. Methods： Samples of ejaculated sperm from idiopathic asthenozoospermia patients and normozoospermic men were separated and purified by Percoll discontinuous density gradients, the distribution and expression of SEPT4 in the sperm samples were determined by im- munocytochemistry, and the expressions of SEPT4 mRNA and SEPT4 protein were detected by RT-PCR and Western blot. Results： Immunocytochemistry showed that the expression of SEPTg, located in the annulus, was significantly reduced in the sperm of the idiopathic asthenozoospermia patients （t = 3. 452, P 〈 0.01 ）. RT-PCR revealed that the expression of SEPT4 mRNA was significantly lower in the sperm of the idiopathic asthenozoospermia patients than in those of the normozoospermic men （ t = 3. 521, P 〈 0.05 ）. Western blot confirmed the results of RT-PCR （t = 5. 872, P 〈 0. 05 ）. Conclusion ： The expression of SEPT4 is significantly de- creased in the ejaculated sperm of idiopathic asthenozoospermia patients, which might be one of the causes of idiopathic asthenozoospermia.

关 键 词：SEPT4蛋白 特发性弱精子症 精子环 逆转录-聚合酶链反应 免疫印迹 

分 类 号：R698.2[医药卫生—泌尿科学]