中华绒螯蟹嗜水气单胞菌微胶囊口服疫苗制备工艺及特性研究  

Preparation technology and characterization of microencapsulated oral vaccine of Aeromonas hydrophila from Eriocheir sinensis

作　　者：李义[1] 刘永贵[1] 俞泉宇[1] 郭明凯[1] 

机构地区：[1]苏州大学医学部基础医学与生物科学学院,江苏苏州215123

出　　处：《淡水渔业》2011年第3期77-82,共6页Freshwater Fisheries

基　　金：江苏省普通高校自然科学研究资助项目(KJD2008240001);苏州市应用基础研究计划项目(SYN201019)

摘　　要：以福尔马林灭活的中华绒螯蟹(Eriocheir sinensis)腹水病及抖抖病并发病病原菌嗜水气单胞菌(Aeromonashydrophila)CL99920菌株为芯材和海藻酸钠为壁材,采用乳化法制备嗜水气单胞菌微胶囊口服疫苗。通过单因素试验和正交试验,研究了海藻酸钠浓度、乳化时间、搅拌速度和菌胶比例对嗜水气单胞菌微胶囊包封率和粒径的影响,确定了嗜水气单胞菌微胶囊疫苗的最佳制备工艺。试验结果显示,在本试验条件下,各因素对嗜水气单胞菌包封率的影响程度由大到小依次为:海藻酸钠浓度>菌胶比例>乳化时间>搅拌速度;制备嗜水气单胞菌微胶囊疫苗的最佳工艺是:海藻酸钠浓度2%,菌胶比例1∶6,乳化时间15 min,搅拌速度1000 r/min。以该工艺制备的嗜水气单胞菌微胶囊具有良好的耐酸性和肠溶性,包封率为73.66%,平均粒径为(56.8±1.6)μm。The formalin killed pathogenic bacteria Aeromonas hydrophila CL99920 from Eriocheir sinensis with hydroperitoneum disease or trembling disease was used as core-material and sodium alginate as shell material,and then the microencapsulated oral vaccine of A.hydrophila was prepared by emulsion method.The effects of concentration of sodium alginate,emulsification time,stirring speed and proportion of A.hydrophila and sodium alginate on embedding rate and diameter of A.hydrophila microcapsules were studied with single factor test and orthogonal test,and then the optimum preparation technology of microencapsulated vaccine of A.hydrophila was determined.The results showed that the effects of factors on embedding rate of A.hydrophila from significant to less in order was concentration of sodium alginateproportion of A.hydrophila and sodium alginateemulsification timestirring speed,and the optimal conditions were as follows：concentration of sodium alginate was 2%,proportion of A.hydrophila versus sodium alginate was 1∶6,emulsification time was 15 min,and stirring speed was 1000 r/min under this experimental conditions.This method can prepare microcapsules which have good acid resistance and enteric solubility,with embedding rate of being 73.66% and mean diameter of（56.8±1.6）μm.

关 键 词：嗜水气单胞菌(Aeromonas hydrophila) 海藻酸钠 微胶囊口服疫苗 制备工艺 

分 类 号：S942.5[农业科学—水产养殖]