神经病理性疼痛大鼠海马组织中NF-κB mRNA、iNOS mRNA、nNOS mRNA表达及意义  

Expressions of NF-κB mRNA,iNOS mRNA,nNOS mRNA in hippocampus in rats with neuropathic pain

作　　者：张丽[1] 谭海波[2] 孙涛[3] 傅志俭[3] 于灵芝[2] 

机构地区：[1]首都医科大学附属北京友谊医院,北京100050 [2]山东大学附属济南市中心医院 [3]山东大学附属省立医院

出　　处：《山东医药》2011年第27期1-3,共3页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(30801072);山东省自然科学基金资助项目(Q2007C14)

摘　　要：目的探讨神经病理性疼痛大鼠模型海马核因子-κB(NF-κB)mRNA、诱导型一氧化氮合酶(iNOS)mR-NA和神经元型一氧化氮合酶(nNOS)mRNA表达及意义。方法将70只雄性Wister大鼠随机分为实验组和对照组各35只,于慢性坐骨神经挤压性损伤(CCI)术前1 d及术后1、47、1、42、1、28 d测定机械痛阈及热痛阈后处死大鼠,取海马组织,采用RT-PCR法测定NF-κB mRNAi、NOS mRNA、nNOS mRNA水平。结果与对照组及术前比较,实验组术后各时点术侧机械痛阈及热痛阈明显降低;NF-κB mRNA水平于术后4 d开始增加,7 d达到高峰,术后14、28 d仍维持于较高水平(P<0.05);iNOS mRNA水平于术后4 d开始增加,nNOS mRNA水平于术后7 d开始增加,两者均在21 d达到高峰,在28 d仍维持在较高水平(P<0.05)。结论 CCI参与了神经病理性疼痛的维持,其机制可能为海马组织NF-κB活化并上调iNOS和nNOS表达。Objective To analyze the expressions of nuclear factor-κB （NF-κB） mRNA, inducible nitric oxide synthase （iNOS） mRNA and neuronal nitrie oxide synthase （nNOS） mRNA in hippecampus in neuropatldc pain model of rats. Methods Seventy male Wister rats were randomly divided into experimental group and observed group with 35 in each, the former received the chronic constriction injury （CCI） and the later received the sham operation. The mechanical and thermal nociceptive thresholds were assessed at 1 d before and 1 d, 4 d, 7 d, 14 d, 21 d and 28 d after CCI operation. Rats were killed and hippocampus were removed at each time point and the expression of NF-KB mRNA, iNOS mRNA and nNOS mRNA in ldppocampus were examined by RT-PCR technique. Results Compared with that of the sham group and before operation, the thresholds to mechanical and thermal stimuli of the experimental group decreased obviously after CCI operation. The expression of NF-KB mRNA in file hippocamplLs began to increase at 4 d after operation, reached the peak at 7 d and maintained at a high level during the 28-day-observation （ P 〈 0.05 ）. The expression of iNOS mRNA began to increase at 4 d after operation and nNOS mRNA began to increase at 7 d （ P 〈0 05）. Both of them reached the peak at 21d and maintained at a high level during the 28-day-observation. Conclusions The activation of NF-KB and the upregu lation of iNOS and nNOS in hippocampus may be involved in the procession of neuropathic pin.

关 键 词：神经病理性疼痛 海马 核因子-ΚB 一氧化氮合酶 

分 类 号：R614[医药卫生—麻醉学]