转DREB1A基因多年生黑麦草T_1代萌发期耐盐性研究  

A study on salt tolerance of T_1 generation transgenic perennial ryegrass with DREB1A geneat germination period

作　　者：包永霞[1] 李雪 满达[3] 韩烈保[1,4] 

机构地区：[1]北京林业大学草坪研究所,北京100083 [2]北京鼎国昌盛生物技术有限责任公司,北京100083 [3]北京林业大学总务与产业管理处,北京100083 [4]长江大学园艺园林学院,湖北荆州434025

出　　处：《草业科学》2011年第8期1440-1444,共5页Pratacultural Science

基　　金：北京林业大学研究生科技创新专项计划(BLYJ201101);北京市重点学科专项

摘　　要：对转DREB1A基因多年生黑麦草(Lolium perenne)的T1代种子和多年生黑麦草非转化植株的子一代种子进行了不同质量分数的NaCl(0、0.3%、0.6%、0.9%、1.2%、1.5%)处理,研究了转基因植株对盐胁迫的生理响应。结果表明,随着盐质量分数递增,相对发芽势、相对发芽率、胚芽长、叶绿素含量、叶片含水量及相对含水量呈现下降的趋势,转基因组与对照组之间的差异达到显著水平,高盐胁迫下,前者各指标均高于后者;胚根长变化无明显规律;细胞膜透性的变化与盐质量分数的增加成正相关,转基因组的细胞膜透性低于对照组,二者存在显著差异。说明转基因植株的耐盐性明显强于对照组,转DREB1A基因多年生黑麦草T1代种子可以作为盐害土壤改良和利用的植物资源。The seeds of transgenic perennial ryegrass （Loliurn perenne） with DREBIA gene were treated with various concentrations of NaC1 （0, 0.3%, 0.6%, 0.9%, 1.2%and 1.5%） to study physiological re sponses of transgenic plants under salt stresses. The results showed that the seed relative germination potential, relative germination rate, seedling height, leaf chlorophyll content, leaf water content and relative water content （RWC） decreased when NaC1 concentration increased. There were significant differences be tween the transgenic group and control group. Variation of root length was not obvious. The leaf relative conductivity had a positive relationship with the increase of salt concentration and with significant differ ences between transgenic plants and control plants. The salt tolerance of transgenic plants improved com- pared to nontransgenic plants. Transgenic T1 generation seeds of perennial ryegrass with DREB1A gene can be used as seed resources for salt soil improvement.

关 键 词：多年生黑麦草 DREB1A基因 T1代 萌发期 耐盐性 

分 类 号：S543.6[农业科学—作物学]