大孔吸附树脂对竹叶兰中总黄酮的分离纯化  

Study on Separation and Purification of Total Flavones from Arundina graminifolia by AB-8 Macroporous Adsorption Resin

作　　者：李晓芬[1] 周学进[1] 张公信[1] 付艳丽[1] 高云涛[1] 

机构地区：[1]云南民族大学化学与生物技术学院,昆明650031

出　　处：《湖北农业科学》2011年第14期2939-2942,共4页Hubei Agricultural Sciences

基　　金：云南省社会发展科技计划项目(2007B148);云南省教育厅科学基金资助项目(06Y195C);国家民委-教育部共建民族药资源化学重点实验室开放基金项目(MJY07010)

摘　　要：大孔吸附树脂分离纯化竹叶兰总黄酮的最佳工艺条件为上样液浓度2.50 g/L,上样速率3.0 BV/h,洗脱剂80%乙醇,洗脱速率3.0 BV/h,洗脱剂用量4.0 BV,按此工艺条件纯化后的竹叶兰总黄酮纯度达81.58%。AB-8型大孔吸附树脂对竹叶兰总黄酮有较好的吸附和解吸效果。The separation and purification of total flavones from Arundina graminifolia by AB-8 macroporous adsorption resin was investigated.The optimum colum conditions were as follows the concentration and current velocity of the original solution was 2.50 g/L and 3.0 BV/h respectively;the eluant was 80% ethanol;the eluting velocity was 3.0 BV/h,and the consumption of eluant was 4BV respectively.The purity of total flavones reached up to 81.58% under these conditions.The maximum adsorbing capacity for total flavonids in Arundina graminifolia was 16.94 g/L.The experiment results showed that AB-8 macroporous adsorption resin was fit for adsorbing and separating the total flavones from Arundina graminifolia.

关 键 词：大孔吸附树脂 总黄酮 分离纯化 竹叶兰 

分 类 号：S567.2[农业科学—中草药栽培] R284.2[农业科学—作物学]