补肾益气活血方对胎儿生长受限胎鼠补肾益气活血方对胎儿生长受限胎鼠脑胰岛素样生长因子-Ⅰ与胰岛素样生长因子结合蛋白-3表达的影响  

Effect of Reinforcing Kidney Replenishing Qi and Promoting Blood Circulation Recipe on the Expression of IGF-Ⅰ and IGFBP-3 in Brain of the Fetal Rat with FGR

作　　者：王琪[1] 熊丽萍[2] 屠庆年[3] 

机构地区：[1]华中科技大学同济医学院附属同济医院中西医结合研究所,武汉430030 [2]广州军区武汉总医院病理科,430070 [3]华中科技大学同济医学院附属同济医院中西医结合科,武汉430030

出　　处：《医药导报》2011年第8期975-978,共4页Herald of Medicine

基　　金：国家自然科学基金资助项目(基金编号:39870923)

摘　　要：目的观察补肾益气活血方对胎儿生长受限(fetal growth restriction,FGR)胎鼠脑胰岛素样生长因子Ⅰ(insulin-like growth factorsⅠI,GF-Ⅰ)、胰岛素样生长因子结合蛋白-3(IGF-binding proteinsI,GFBP-3)表达的影响,探讨补肾益气活血方治疗FGR的作用机制。方法采用被动吸烟法建立SD大鼠FGR模型,分为正常组、FGR组、补肾益气活血方治疗组(中药组)、精氨酸治疗组(精氨酸组)。采用免疫组织化学方法检测胎鼠脑IGF-ⅠI、GFBP-3的表达。结果与FGR组[IGF-ⅠI、GFBP-3吸度度值分别为(0.148 3±0.015 6)(0.168 9±0.021 4)]比较,中药组胎鼠脑IGF-Ⅰ、IGFBP-3的表达([0.191 3±0.017 8)(,0.187 5±0.023 3)]均明显升高(P<0.05),IGF-Ⅰ表达强度高于精氨酸组[(0.193 3±0.020 3)(,P<0.05);]中药组与正常组比较,差异无统计学意义(P>0.05)。结论补肾益气活血方可能通过调节IGF-ⅠI、GFBP-3表达,促进胎鼠在宫内的生长发育。Objective To investigate the effect and mechanism of reinforcing kidney, replenishing qi and promoting blood circulation recipe on the protein expression of IGF-I and IGFBP-3 in the brains of rat fetal growth restriction （FGR）. Methods The FGR model was created by passive smoking in SD rats . Thirty-two pregnant SD rats were randomly divided into four groups： control group, FGR group, FGR rats treated with reinforcing kidney, replenishing qi and promoting blood circulation recipe （Chinese medicine group）, FGR rats treated with L-arginine （L-arginine group）. The protein expression of IGF-I and IGFBP-3 in the fetal brain was analyzed by immunohistochemistry. Results Compared with the FGR model controls [ （0. 148 3±0.015 6） and （0. 168 9±0.021 4） ], the expression of IGF-I and IGFBP-3 in fetal brains of Chinese medicine treated group [ （0. 191 3± 0. 017 8 ） and （0. 187 ±0.023 3 ） ] increased significantly（ P〈0.05 ） ; in addition, the expression of IGF-I was higher than that of L-arginine treated group [ （0. 193 3±0.020 3 ） ] as well （P〈0.05）. The difference between the Chinese medicine treated group and the model control one was insignificant （P〉0.05）. Conclusion The recipe of reinforcing kidney, replenishing qi and promoting blood circulation recipe promotes fetal growth in uterus by regulating the expression of IGF-I and IGFBP-3.

关 键 词：补肾益气活血方 胎鼠 胎儿生长受限 胰岛素样生长因子-Ⅰ 胰岛素样生长因子结合蛋白-3 

分 类 号：R977[医药卫生—药品] R965[医药卫生—药学]