微柱凝胶法检测ABO母婴血型不合孕妇血清抗-A(B)IgG效价1154例的结果分析  

Analysis of results of serum anti-A(B) IgG titer detection in 1 154 cases maternal-fetal ABO blood group incompatible pregnant women by microcolumn gel technology

作　　者：廖思红[1] 邹勇[1] 袁青[1] 覃雪玲[1] 刘文达[1] 

机构地区：[1]中山大学附属第三医院,510630

出　　处：《实用医技杂志》2011年第8期805-807,共3页Journal of Practical Medical Techniques

摘　　要：目的探讨微柱凝胶法(MGT)检测1 154例ABO母婴血型不合孕妇血清抗-A(B)IgG效价的临床意义。方法采用MGT法对1 154例ABO母婴血型不合孕妇进行产前血清抗-A(B)IgG效价测定,分析血清抗-A(B)IgG效价与新生儿溶血病(HDN)发生的相关性。结果 1 154例孕妇在孕20周首次血清抗-A(B)IgG效价测定≥1∶64者占72.36%(835/1 154),其中O-A型、O-B型和O-AB型夫妇抗-A(B)IgG效价的阳性率分别为72.48%(366/505)、66.45%(313/471)和80.90%(144/178),各组阳性率差异有统计学意义(χ2=13.765,P<0.01)。在产前第30周左右对1 154孕妇中的340例进行抗-A(B)IgG效价的二次随机测定,发现50.50%(51/101)孕妇抗体效价从阴性转为阳性,51.80%(129/249)孕妇(效价≥1∶64)抗体效价继续上升,其中O-A型、O-B型和O-AB型夫妇抗-A(B)IgG效价上升率分别为53.74%(79/147)、50.00%(77/154)和56.41%(23/39),各组间差异无统计学意义(χ2=1.130,P>0.05);25例发生HDN的患儿母亲产前抗-A(B)IgG效价测定均≥1∶256,其中有12例血型抗体效价持续升高达4倍以上,1例效价<1∶64。结论 ABO母婴血型不合孕妇血清抗-A(B)IgG效价水平与HDN的发生显著相关,利用MGT检测孕妇血清抗-A(B)IgG效价有助于HDN的早期诊断和干预。Objective To investigate the clinical significance of detection the titer of serum anti-A（B） IgG in 1 154 ABO blood group incompatible pregnant women by microcolumn gel technology（MGT）.Methods Detect the titer of serum anti-A（B） IgG in 1 154 cases ABO incompatible pregnant women,and evaluate the relationship between the titer of serum anti-A（B） IgG and hemolytic disease of newborn（HDN）.Results 72.36% of total 1 154 cases ABO incompatible pregnant women for the first time detection at 20 weeks′ pregnant have the titer of serum anti-A（B） IgG higher than 1∶64,in which the positive rate in O/A,O/B and O/AB type couple group account for 72.48%（366/505）,66.45%（313/471） and 80.90%（144/178）,respectively.There was statistically significant difference between the positive rate of each groups（χ2=13.765,P0.01）.The titer of serum anti-A（B） IgG in 340 cases pregnant women in 1 154 at about 30 weeks＇ pregnant was randomly detected for the second time.It was found that the titer of serum anti-A（B） IgG in 50.50%（51/101） pregnant women was transformed from negative to positive,and the titer in 51.80%（129/249） pregnant women was increased continuously,in which the rate of rise of 3 groups of O/A,O/B and O/AB types were 53.74%（79/147）,50.00%（77/154） and 56.41%（23/39）,respectively.There was no statistically significant among 3 groups（χ2=1.130,P0.05）.In mothers of 25 cases newborns with HDN,the titer of serum anti-A（B） IgG of 25 cases who had newborn with HDN was higher than 1∶256 and of which the titer of that of 12 cases increased more than 4 times.Only one case,the titer was less than 1∶64.Conclusion There was a significant relationship between the titer of serum anti-A（B） IgG in ABO blood group incompatible pregnant women and HDN.

关 键 词：血型不合 幼红细胞增多症 胎儿 血清学试验 微柱凝胶法 

分 类 号：R446.6[医药卫生—诊断学]