猪DAZ基因的DNA池测序分析  

Analysis of Parts of Pig DAZ Gene by DNA Pooling and Sequencing

作　　者：李敬瑞[1] 丁远华 向程举 刘若余[1] 

机构地区：[1]贵州大学动物科学学院高原山地动物遗传育种与繁殖教育部重点实验室,贵州贵阳550025 [2]毕节地区种畜场,贵州毕节551700 [3]毕节地区畜牧技术推广站,贵州毕节551700

出　　处：《中国畜牧兽医》2011年第8期60-62,共3页China Animal Husbandry & Veterinary Medicine

基　　金：贵州省农业科技攻关项目[黔科合NY字(2009)3073]

摘　　要：选取大约克猪、白香猪、可乐猪3个猪种的公猪构建品种DNA池。对DAZ基因的部分CDS区和3′UTR进行PCR扩增,并对PCR产物进行测序。结果表明,3个猪种中,DAZ基因的该序列未发现任何突变。同源性分析结果表明,猪与人、猩猩、猴、鼠、牛的DAZ基因该序列核苷酸同源性分别为97.0%、95.2%、96.1%、92.9%、97.0%。说明在哺乳动物中,其DAZ基因CDS部分序列和3′UTR是高度保守的。系统进化分析结果表明,猪和牛在一个进化支上。蛋白疏水性分析表明,在17-20位有个强疏水区。Three breeds of male pigs（Coke pig,Yorkshire and White pig） were applied to sequence in parts of CDS,3′UTR of DAZ gene based on DNA pooling and PCR products.The results showed that DAZ gene were found mutations in the three pig breeds.The results of homologous analysis showed that the homologous rate of nucleotide sequences of the parts of CDS,3′UTR in human,orangutan,monkey,mouse and cow were 97.0%,95.2%,96.1%,92.9% and 97.0%,respectively.It showed that the parts of CDS and 3′UTR of the DAZ gene sequence in mammals were highly conservaton.Pigs could be used to establish animal mode,providing scientific foundation.Phylogenetic analysis showed that pig and cow were in a branch on the evolutionary.The analysis of the protein hydrophobicity showed that a strong hydrophobic was in the 17 to 20.

关 键 词：DAZ基因 DNA池 测序 分析 

分 类 号：Q78[生物学—分子生物学]