犬瘟热病毒贵州分离株N基因的克隆及序列分析  

Cloning and Sequence Analysis of N Gene of Canine Distemper Virus Isolated from Guizhou

作　　者：周莉[1] 刘志杰[1] 曾智勇[1,2] 汤德元[1] 李谦[1] 王彬[1] 张晓杰[1] 甘振磊[1] 王凤[1] 

机构地区：[1]贵州大学动物科学学院,贵州贵阳550025 [2]贵州省动物疫病研究室,贵州贵阳550025

出　　处：《中国畜牧兽医》2011年第8期63-66,共4页China Animal Husbandry & Veterinary Medicine

基　　金：贵州大学博士基金(X060054)

摘　　要：根据GenBank中发表的犬瘟热病毒Onderstepoort株N蛋白基因序列设计两对特异性引物,采用RT-PCR扩增犬瘟热病毒贵州分离株(CDV-GZ1)的N基因,并进行克隆与序列分析。结果显示,CDV-GZ1株N基因的ORF全长1572bp,其编码氨基酸序列与国外Shuskiy株和01-2689株的同源性分别为98.9%和97.1%,与部分国内分离株同源性在95%以上,说明N蛋白是保守性较强的结构蛋白。Two pairs of primers were designed and synthesized based on the sequence of the Onderstepoort strain of canine distemper virus reported in GenBank,and the N gene was amplified by reverse transcription polymerase chain reaction（RT-PCR） from CDV-GZ1 strain in Guizhou.The amplified fragment was cloned and analyzed.The results showed that the length of N gene was 1572 bp.The homology of coding amino acid between this strain and CDV Shuskiy and 01-2689 strain was 98.9% and 97.1% respectively,while above 95% among other domestic strain.It illustrated that N protein have highly conservation property.

关 键 词：犬瘟热病毒 N蛋白 克隆 序列分析 

分 类 号：Q78[生物学—分子生物学]