犬瘟热病毒分离株N、H、F蛋白基因的变异分析  

Genetic Diversity Analysis of the N,H,F Protein Genes of Canine Distemper Virus Isolate

作　　者：王旭荣[1] 王小辉[1] 张世栋[1] 李世宏[1] 潘虎[1] 严作廷[1] 

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所中国农业科学院临床兽医学研究中心,甘肃兰州730050

出　　处：《中国畜牧兽医》2011年第8期71-77,共7页China Animal Husbandry & Veterinary Medicine

基　　金：中央级公益性科研院所基本科研业务费专项资金项目(BRF080302)

摘　　要：通过RT-PCR方法扩增到犬瘟热病毒(canine distemper virus,CDV)分离株GS0812-4的N、H、F蛋白基因,然后克隆入pGEM-T Easy载体并进行序列分析和抗原表位预测分析。结果表明,GS0812-4分离株的N、H、F蛋白基因与其他分离株相应序列的核苷酸同源性分别为93.2%～97.8%、90.3%～97.3%、93.2%～97.8%,其推定的氨基酸同源性分别为96.6%～98.7%、91.3%～97.9%和96.6%～98.7%;GS0812-4分离株N、H、F蛋白基因与疫苗株相应序列的核苷酸同源性分别为93.1%～93.8%、89.4%～90.5%、93.1%～93.8%,其推定的氨基酸同源性分别为96.4%～97.3%、89.3%～91.3%、96.4%～97.3%。由基因系统发育树可知,GS0812-4与疫苗株处于不同的分支,其中与MKY-KM08的亲缘关系最近,但GS0812-4在N、H、F 3个基因系统发育树上与其他同一毒株的亲缘关系远近不同。H、F蛋白的抗原表位预测结果表明,GS0812-4与MKY-KM08和疫苗株的抗原表位均有差异。说明GS0812-4是野毒株,与MKY-KM08属于同一基因型,来源于同一毒株,但两毒株间的H蛋白和F蛋白的抗原表位仍有差异。The nucleoprotein（N）,haemgagglutinin protein（H）and fusion protein（F） genes of canine distemper virus（CDV） isolate GS0812-4were obtained by RT-PCR and sequenced after being cloned directly into the pGEM-T Easy.Thesequences of N,H and F genes of GS0812-4 were compared with sequences and antigen epitope of other CDV strains in GenBank by BLAST and DNAStar software.The results showed that the N,H and F of GS0812-4 shared 93.2% to 97.8%,90.3% to 97.3%,93.2% to 97.8% of nucleotide homology,and 96.6% to 98.7%,91.3% to 97.9% and 96.6% to 98.7% of amino acid identity with that of referenced strains,respectively.However,The N,H and F genes of the GS0812-4 shared only 93.1% to 93.8%,89.4% to 90.5% and 93.1% to 93.8% of nucleotide homology,and 96.4% to 97.3%,89.3% to 91.3% and 96.4% to 97.3% of amino acid homology with that of vaccine strains,respectively.Phylogenetic tree showed that GS0812-4 was different from the vaccine strain of the branch,it had a recent genetic relationship and MKY-KM08.But GS0812-4 compared with other strains of different genetic distance relationships.H,F protein epitope prediction results showed that GS0812-4 epitope was different from and MKY-KM08 and vaccine strains.Results showed that GS0812-4 was the wild strain,and the GS0812-4 strain was MKY-KM08 belonged to the same genotype,derived from the same strain,but there were still differences between epitopes.

关 键 词：犬瘟热病毒 H蛋白 H蛋白 F蛋白 变异分析 

分 类 号：Q78[生物学—分子生物学]