转化生长因子β对成骨细胞增殖、BMP-2及Cbfal基因表达的影响  

Effect of transformation growth factor beta (TGF-β) on the proliferation,bone morphogenetic protein-2 (BMP-2) and core binding factor al (Cbfal) gene expression in cultured rat osteoblast

作　　者：王惠娟[1] 董进[1] 

机构地区：[1]山西医科大学第一临床医学院内分泌科,山西太原030001

出　　处：《中国当代医药》2011年第22期21-23,共3页China Modern Medicine

摘　　要：目的:通过转化生长因子β(TGF-β)对体外成骨细胞增殖、骨形态蛋白-2(BMP-2)及核心结合因子1(Cbfal)基因表达的影响,探讨TGF-β对骨代谢影响的可能机制。方法:不同浓度的TGF(0、0.01、100ng/ml)刺激体外培养的大鼠成骨细胞,采用噻唑蓝(MTT)法检测细胞增殖能力,用半定量RT-PCR法检测成骨细胞BMP-2、Cbfal基因的表达。结果:TGF-β可明显促进成骨细胞增殖(P<0.05),并促进成骨细胞BMP-2、Cbfal基因的表达(P<0.01),具有浓度依赖性。结论:TGF-β可促进成骨细胞的增殖、分化及成熟,可能是通过增强BMP-2、Cbfal基因的表达来实现的。Objective： To study the effect of TGF-βo n the proliferation, BMP-2 and Cbfal gene expression in rat osteoblast in vitro, discusses the influence of TGF-β on bone metabolism can influence mechanism. Methods： Cultured rat osteoblast were treated with TGF-β in different concentrations （0、0.01、100 ng/ml）. Cell proliferation was assessed by MTT, coiorimetric assay, the expression of BMP-2, Cbfal gene in rat osteoblast were examined by semiquantitive RT-PCR.Results： TGF-β could promote osteoblast proliferation （P0.05） and increase the expression of BMP-2 and Cbfal gene in a does-dependent manner（P0.01）. Conclusion： TGF-β induces the proliferation differentiation and maturity of osteoblast probably by increasing the expression of BMP-2 and Cbfal gene.

关 键 词：转化生长因子Β 成骨细胞 增殖 骨形态蛋白-2 核心结合因子-1 

分 类 号：R318[医药卫生—生物医学工程]