绒毛组织二氢二醇环氧苯并（a）芘-DNA加合物与孕早期胚胎停育  

Relationship between villus BaP-DNA adducts level and blighted ovum in early pregnancy

作　　者：侯海燕[1] 杨振华[1] 邹晓萍[1] 陈亚琼[1] 

机构地区：[1]中国人民武装警察部队医学院附属医院妇产科,天津300162

出　　处：《中华围产医学杂志》2011年第8期459-462,共4页Chinese Journal of Perinatal Medicine

基　　金：天津市自然科学基金（07JCZDJC07400）

摘　　要：目的研究绒毛组织二氢二醇环氧苯并（a）芘-DNA加合物与孕早期胚胎停育之间的关系，探讨影响胚胎发育的可能环境因素。方法选择门诊诊断胚胎停育的妊娠妇女102例（病例组），同期按年龄、孕产次、妊娠时间进行1：1配比，选取正常妊娠要求终止妊娠的妇女102例（对照组），人工流产术后留取绒毛组织，生理盐水冲洗后匀浆，提取基因组DNA并做浓度定量，高效液相色谱法检测绒毛BaP—DNA加合物的浓度，问卷调查获取母亲个人资料，Logistic回归分析绒毛组织BaP-DNA加合物与胚胎停育的关系。结果病例组绒毛组织加合物水平显著高于对照组[（8．9±8．2）加合物／10。核苷酸与（2．0±1．4）加合物／10^8核苷酸，P〈0．05]。绒毛组织BaP—DNA加合物的浓度越高，孕妇发生胚胎停育的危险性越大，当加合物浓度超过6．06加合物／10。核苷酸，发生胚胎停育的危险性将增加到59．39倍（95％CI：15．50～227．55）。控制可能的混杂因素后，发现孕妇受教育程度在高中及高中以上为胚胎停育的保护因素（OR=-0．21，95％CI：-0．19～-0．03）。结论绒毛组织中较高浓度的BaP—DNA加合物可能会增加孕早期胚胎停育发生的危险性，对胚胎发育可能有不良影响。Objective To investigate relations between villus Benzo （a）pyrene （BaP） DNA adducts and blighted ovum in early pregnancy, and to explore possible environmental factors influencing embryo development. Methods One hundred and two pregnant women with blighted ovum were selected into this study as research group; and 102 normal pregnant women were taken as control group; the age, gravidity, parity and gestational weeks of the two groups were matched. After artificial abortion, villi of the patients were collected and washed by normal saline. Then, the tissue was homogenated and genome DNA was extracted to detect quantity of the tissue. BaP-DNA adducts levels were examined by high-performance liquid chromatography-fluorescence method （HPLC）. The personal information of pregnant women was collected by questionnaire. Logistic regression model was used to investigate the association between BaP-DNA adducts and blighted ovum. Results BaP-DNA adducts level in villi of research group [（8. 9±8. 2） adducts/108 nucleotides] was significantly higher than that of control group [-（2.0±1.4） adducts/10^8 nucleotides], P〈0.05. The higher the BaP-DNA adducts in villi, the higher risk the blighted ovum; when the BaP-DNA adducts level increased to 6.06 adducts/10^8 nucleotides, the risk of blighted ovum might increase 59.39 times （95% CI：15. 50-227. 55）. Maternal education level was a protective factor （OR：=0.21, 95%CI：-0.19-0.03） after controlling potential confounders. Conclusions High level of villi BaP-DNA adducts might increase the risk of blighted ovum in early pregnancy, and could have an adverse effect on embryo development.

关 键 词：胚胎发育 妊娠初期 绒毛膜绒毛 苯并芘 DNA加合物 色谱法 高压液相 

分 类 号：R714.2[医药卫生—妇产科学]