复方当归注射液可减轻大剂量柴油机尾气颗粒物对雌性小鼠卵和卵裂球的损伤  

Compound radix angelicae sinensis injection improves oocyte and segmentation sphere injury resulted from subacute exposure to heavy diesel exhaust particles in female mice

作　　者：洪新如[1] 方芳[1] 宋岩峰[1] 余宏宇[2] 赵蕾[1] 孙庆华[3] 

机构地区：[1]南京军区福州总医院妇产科,福州350025 [2]第二军医大学附属长征医院病理科 [3]美国俄亥俄州立大学公共卫生学院环境健康科学系

出　　处：《中华围产医学杂志》2011年第8期475-482,共8页Chinese Journal of Perinatal Medicine

基　　金：南京军区医学科技资助项目（07M093）;福建省科技厅计划重点项目基金（2007Y0017）;美国健康影响研究院基金（4747.RFPA05-3/06-8）

摘　　要：目的观察雌性小鼠亚急性接触柴油机尾气颗粒物（diesel exhaust particles，DEP）致卵和卵裂球损伤以及复方当归注射液对损伤的干预效果。方法210只21dICR雌鼠随机分为对照组（A组）、DEP组（B组）、DEP＋小剂量组（C组）、DEP＋中剂量组（D组）和DEP＋大剂量组（E组）（各42只），分别向小鼠咽后壁接种12．0μg／μlDEP混悬液（B～E组）或载液（PBS，A组）各30μl，每3d重复接种1次，共4次。末次DEP接触后3d处死动物。C、D、E组首次接种日至处死前1d，每日分别经腹腔给予复方当归注射液相当于生药75、150和300mg各1次，连续给药12次。观察指标包括小鼠一般情况、体重；卵巢重量、卵巢重量／体重比值、卵巢组织超氧化物歧化酶（superoxide dismutase，SOD）活性、还原型谷胱甘肽（reduced glutathione，GSH）和丙二醛（malonaldehyde，MDA）含量；卯母细胞的存活率、胚泡破裂比率、第一极体释放率、受精率、线粒体DNA含量和超微结构变化。结果（1）各组小鼠体重差异无统计学意义（P〉0．05）。卵巢重量、卵巢重量／体重比值、卵巢SOD活性和GSH含量B组分别为（1．5±0．6）mg、（7．2±2．5）×10^-5、（192．10±23．67）nU／mgprot和（262．40±31．60）nmol／mgprot，C组分别为（1．7±0．2）mg、（8．9±0．6）×10^-5、（198．92±24．27）nU／mgprot和（271．66±14．58）nmol／mgprot，D组分别为（2．1±0．2）mg、（9．8±1．1）×10^-5、（214．37±27．19）nU／mgprot和（285．93±9．55）nmol／mgprot，低于A组，分别为（3．3±1．5）mg、（15．4±7．3）×10^-5、（292．30±40．03）nU／mgprot和（367．98±24．59）nmol／mgprot（P〈0．05或〈0．01）；E组分别为（3．7±1．1）mg、（18．7±5．4）×10^-5、（279．10±12．63）nU／mgprot和（353．59±10．61）nmol／mgprot，显著高于B组（P〈0．01）；MDA含量B、C、D组分别为（3．88±0．35�Objective To investigate the effects of compound radix angelicae sinensis injection on oocyte and segmentation sphere injured by subacute exposure to diesel exhaust particles （DEP） in female mice. Methods Two hundred and ten 21-day-old ICR female mice were randomly divided into 5 groups： the control group （group A）, DEP group （group B）, DEP±low-dose group （group C）, DEp±middle-dose group （group D） and DEP±high-dose group （group E）. There were 42 mice in each group. The mice were inoculated with 30 μ1 DEP suspension at 12.0μg/μl （group B-E） or the same volume of vehicle （PBS, group A） on pharynx posterior wall by sample pipettor beginning at day 21 and repeated every 3 days for 4 times. The mice were sacrificed three days after the last exposure. Compound radix angelicae sinensis injection containing 75 （group C）, 150 （group D） and 300 （group E） grams of crude drug, respectively, which was intraperitoneally administered for each mouse daily from the day of the first DEP inoculation till the day before sacrifice, consecutively for 12 days. The general conditions were observed, and the body weight and ovary/body weight ratio were tested. Superoxide dismutase （SOD） activity, malondialdehyde （MDA） and reduced glutathione （GSH） contents in ovarian tissues were assayed. Rates of survival, germinal vesicle breakdown, extrusion of the first polar body and in-vitro fertilization, and quantity of mitochondrial DNA for oocytes were investigated. Ultrastructural changes of oocytes were observed. Results （1） No significant difference of the body weight was found among all the groups （P〉0.05）. The ovary weight, ovary/body weight ratio, ovary SOD and GSH content were significantly decreased in groups B[（1.5 ± 0.6） mg, （7.2 ± 2.5） ×10^-5 , （192.10±23.67） nU/mg prot and （262.40±31.60） nmol/mg prot], and C [（1.7±0.2） mg, （8.9±0.6）×10^-5, （198.92±24.27） nU/mg prot and （271.66±14.58） nmol/mg prot] and D E（2. 1±0. 2�

关 键 词：丹参注射液 复方合剂 车辆排放物 颗粒物 卵母细胞 小鼠 

分 类 号：R285.5[医药卫生—中药学]