周围神经损伤后碱性成纤维细胞生长因子mRNA表达的实验研究  

Experimental Study on Dynamic Changes of Expression of Basic Fibroblast Growth Factor mRNA after Peripheral Nerve Injury

作　　者：池雷霆[1] 裴福兴[1] 王光林[1] 杨均[1] 夏庆杰[1] 丁兰[1] 杨静[1] 

机构地区：[1]华西医科大学附属第一医院,成都610041

出　　处：《中华创伤杂志》1999年第6期407-409,共3页Chinese Journal of Trauma

基　　金：国家自然科学基金!资助项目(No .39670729)

摘　　要：目的　研究大鼠坐骨神经钳夹伤后碱性成纤维细胞生长因子（ｂＦＧＦ）ｍＲＮＡ表达的变化规律。方法　１０２ 只Ｗｉｓｔａｒ 大鼠随机分为正常组６ 只，对照及实验组各４８ 只，用持针钳钳夹坐骨神经，于伤后４ 小时、１，３，７，１０，１４，２１，２８ 天采用逆转录－ 聚合酶链反应技术（ＲＴ－ ＰＣＲ） 结合聚丙烯酰胺凝胶电泳及ａｌｐｈａ－３２ｐ－ ｄＣＴＰ放射自显影，检测大鼠坐骨神经钳夹伤局部、腰４ ～６ 背根节及相应脊髓节段ｂＦＧＦｍＲＮＡ的动态变化。结果　实验组伤后４ 小时ｂＦＧＦｍＲＮＡ在损伤局部、脊髓及背根节的表达均开始增强，分别为１．４１±０．１０，１．７８±０ ．１０ 和２．１７±０．１２；伤后７ 天表达值最高，分别为２．７２±０．１４，３ ．６５±０．１７ 和３．９０ ±０．０６；伤后２８ 天表达值为１．２３ ±０．０６ ，１．４３±０ ．０５，１ ．７８±０．０３，降至正常及对照组水平（Ｐ＞ ０．０５）。Objective To study mRNA expression of basic fibroblast growth factor(bFGF) after crushing injury of sciatic nerve in rats. Methods One hundred and two Wistar rats were randomized into a normal group( n =6), a control group ( n =48) and a test group ( n =48). Sciatic nerve was crushed for 5 minutes using modified foceps. Reverse-polymerase chain reaction(RT-PCR) with polyacrylamide gel electrophoresis and alpha -32 P-dCTP labelling autoradiography were employed to detect the dynamic changes of bFGF mRNA in the lesion site, dorsal root ganglia of 4th-6th lumbar spine and spinal cord at 4 hours, 1, 3, 7, 10, 14, 21, 28 days after injury, respectively. Results bFGF mRNA expression of the test group began to increase in the lesion site, dorsal root ganglia and spinal cord at 4 hours (1.41±0.10, 1.78±0.10, 2.17±0.12), reached the peak at 7th day (2.72±0.14, 3.65±0.17, 3.90±0.06), returned to control levels at 28th day (1.23±0.06, 1.43 ±0.05, 1.78±0.03) after injury. Conclusions The increase of endogenous bFGF mRNA after peripheral nerve injury may enhance the regeneration of peripheral nerve.

关 键 词：周围神经损伤 BFGF MRNA RT-PCR 

分 类 号：R651.3[医药卫生—外科学]