消瘀片对粥样硬化兔血管壁组织ET-1mRNA和NOSmRNA表达的影响  

Effects of Xiaoyu Tablet on Expression of ET-1 mRNA and NOSmRNA of Vessel Wall in Atherosclerotic Rabbit

作　　者：谢梅林[1] 顾振纶[1] 周文轩[1] 郭次仪[1] 陈可冀[2] 

机构地区：[1]苏州中药研究所,215007 [2]中国中医研究院

出　　处：《中草药》1999年第11期834-837,共4页Chinese Traditional and Herbal Drugs

基　　金：香港保健协会资助

摘　　要：采用原位杂交和ＲＴ－ＰＣＲ方法，观察消瘀片治疗１６周动脉粥样硬化免后对血管壁组织ＥＴ－１ｍＲＮＡ和ＮＯＳｍＲＮＡ表达的影响。结果显示，粥样硬化组的ＥＴ－１ｍＲＮＡ和ｉＮＯＳｍＲＮＡ表达较多，而 ｃＮＯＳｍＲＮＡ表达较少。使用消瘀片后，动脉壁组织中的 ＥＴ－１ｍＲＮＡ表达明显减少，提示血浆和血管壁组织中的 ＥＴ－１减少是由于 ＥＴ－１ｍＲＮＡ转录水平降低所致。消瘀片对血管壁组织中ｉＮＯＳｍＲＮＡ和ｃＮＯＳｍＲＮＡ表达影响是不同的，它可降低ｉＮＯＳｍＲＮＡ表达，增加ｃＮＯＳｍＲＮＡ表达。表明消瘀片可通过调节 ＮＯＳ不同亚型基因的表达而消减动脉粥样硬化病变的程度。Techniques of in situ hybridization and RT-PCR as well as image pattern analysis were used In this study. The results indicated that ET-1 mRNA and iNOSmRNA expression in atherosclerotic model group were obviously increased, while cNOS, mRNA expression was decreased. After treatment with Xiaoyu tablet, a drug containing extracts of Salvia miltiorrhiza Bunge and Crutaegus pinnattifida Bunge for the removal of blood stasis, for 16 weeks, ET-1 mRNA expression of vessel wall was reduced, suggesting that reduction of ET-1 in plasma and vessel wall was due to inhibition of ET-1 mRNA transcription. Effects of Xiaoyu tablets on iNOSmRNA and cNOSmRNA expression were very different, the drug might reduce iNOS mRNA expression and enhance cNOSmRNA expression, indicating that Xiaoyu tablet might abate the atherosclerotic degree through regulating the expression of NOS subgroup genes.

关 键 词：消瘀片 内皮素 NOS MRNA 动脉粥样硬化 

分 类 号：R286.2[医药卫生—中药学] R285.5[医药卫生—中医学]