对氧磷单克隆抗体的研制及鉴定  

Production and identification of anti paraoxon monoclonal antibodies

作　　者：王刚垛[1] 徐勤惠[2] 马兆扬[1] 荣康泰[2] 何凤生[1] 

机构地区：[1]中国预防医学科学院劳动卫生与职业病研究所,北京100050 [2]军事医学科学院药物毒物研究所

出　　处：《卫生研究》1999年第6期321-323,共3页Journal of Hygiene Research

基　　金：国家"九五"攻关基金资助项目

摘　　要：重氮化法使对氧磷（Ｅ６００）与牛血清白蛋白（ＢＳＡ）、血蓝蛋白偶合合成人工抗原。用人工抗原Ｅ６００－ ＢＳＡ免疫Ｂａｌｂ／ｃ小鼠，然后将ＳＰ２／０小鼠骨髓瘤细胞和Ｅ６００－ ＢＳＡ免疫的Ｂａｌｂ／ｃ小鼠脾脏细胞融合，成功的建立了能分泌抗对氧磷单克隆抗体的杂交瘤细胞株。并分析了该细胞株的染色体及对其产生的单克隆抗体的类型、特异性、亲和力进行了鉴定和检测。上述杂交瘤细胞连续传代或冻存１０周。Paraoxon(E600)was conjugated to bovine serum albumin(BSA) or tachypleus tridentatus hemocyanin(TTH) by diazotization. Two hybridoma cell lines secreting monoclonal antibodies(McAb) against paraoxon have been established by fusing mouse myeloma cells and splenocytes from Balb/c mice immunized with E600 BSA.The chromosomes of the hybridoma cell 2B 10 were analyzed. The immunoglobulin of the McAb was classified. The affinity and specificity of this antibody were determinated. The hybridoma cells have fairly reserved the antibody producing capacity after continuously growing or stored in liquid nitrogen for 10 weeks.

关 键 词：对氧磷 人工抗原 单克隆抗体 农药 

分 类 号：R139.3[医药卫生—劳动卫生] R392－33[医药卫生—公共卫生与预防医学]