氯化饮水中有机提取物对大鼠和人肝肿瘤细胞(HepG2)的遗传毒性及脂质过氧化作用  

Genotoxicity and lipid peroxidation caused by organic extracts of chlorinated drinking water in rats and HepG2 cells

作　　者：鲁文清[1] 越飞[1] 陈秀娜[1] 李晓燕[1] 谢虹[1] VolkerMersch-Sundermann CorneliaJenter 

机构地区：[1]同济医科大学环境卫生学教研室,武汉430030 [2]德国海德堡大学曼海姆临床医学系医学微生物和卫生研究所

出　　处：《卫生研究》1999年第6期326-328,共3页Journal of Hygiene Research

基　　金：国家教委留学回国人员启动基金

摘　　要：The genotoxic effects of chlorinated drinking water(CDW) extracts were examined in human HepG2 hepatoma cells, liver cells and polychromatic erythrocytes (PCEs) of bone marrow of Wistar rats by using in vivo and in vitro micronucleus(MN) test and single cell gel electrophoresis(comet assay). The influence of CDW on the lipidperoxidation (LPO) in blood and tissue of rats was also studied. The results indicated a significant increase of the frequencies of MN in PCEs at the low concentration of CDW and in HepG2 at the high concentration of CDW. The increased DNA breakage caused by CDW was observed in rat liver cells and in HepG2 cells by using comet assay. The levels of malon dialdehyde(MDA) were significantly increased almost in all animals and in all organs tested. A dose dependent increases of MDA were observed in rat liver. It was demonstrated that CDW extracts caused genotoxic and oxidative damage on animals.The genotoxic effects of chlorinated drinking water(CDW) extracts were examined in human HepG2 hepatoma cells, liver cells and polychromatic erythrocytes (PCEs) of bone marrow of Wistar rats by using in vivo and in vitro micronucleus(MN) test and single cell gel electrophoresis(comet assay). The influence of CDW on the lipidperoxidation (LPO) in blood and tissue of rats was also studied. The results indicated a significant increase of the frequencies of MN in PCEs at the low concentration of CDW and in HepG2 at the high concentration of CDW. The increased DNA breakage caused by CDW was observed in rat liver cells and in HepG2 cells by using comet assay. The levels of malon dialdehyde(MDA) were significantly increased almost in all animals and in all organs tested. A dose dependent increases of MDA were observed in rat liver. It was demonstrated that CDW extracts caused genotoxic and oxidative damage on animals.

关 键 词：氯化饮水 有机提取物 微核 肝肿瘤细胞 遗传毒性 

分 类 号：R123.5[医药卫生—环境卫生学] R994.6[医药卫生—公共卫生与预防医学]