用全胚胎培养方法评价重铬酸钾对大鼠和小鼠胚胎的发育毒性  

Evaluation of the developmental toxicity of potassium dichromate using whole embryo culture of mouse and rat embryos

作　　者：袁立懋 孙棉龄[2] 吴德生[2] 

机构地区：[1]广州市卫生防疫站毒理科,广州510080 [2]华西医科大学公共卫生学院,成都610041

出　　处：《中国药理学与毒理学杂志》1999年第4期306-309,共4页Chinese Journal of Pharmacology and Toxicology

摘　　要：采用植入后全胚胎培养方法研究重铬酸钾对大，小鼠胚胎的发育毒性． 结果表明，重铬酸钾在１．０ 和２．５ ｍ ｇ·Ｌ－ １以上时分别可致大，小鼠胚胎体长，头长，卵黄囊直径及胚胎干重等各项指标明显低于对照组，同时观察到大，小鼠胚胎脑，神经管和体屈等发育迟缓，组织光镜可见胚胎神经管上皮变薄，细胞排列不规则． 以上结果均呈明显的剂量－反应关系，表明重铬酸钾体外对大，小鼠胚胎具有明显的发育毒性，且大鼠比小鼠敏感．Nine and a half day old rat embryos were explanted and cultured at six different concentrations of potassium dichromate in rat serum solutions (0, 0.25, 0.5, 1.0, 2.0, 4.0 mg·L -1 Cr) for 48 h. Rat embryo development in 0.25 mg·L -1 Cr was not significantly different from the control whereas the higher concentrations of Cr resulted in a concentration related decrease in yolk sac diameter, crown rump length, head length, embryo dry weight and Brown′s morphological developmental score. The 0.5 mg·L -1 Cr and higher concentrations also resulted in embryotoxicity as well as abnormalities, which included neural tube defect, deficient in axial rotation and fore limb, maxillary process defects. Eight and a half day old mouse embryos were explanted and cultured in 0, 0.6, 1.2, 2.5, 5.0, 10.0 mg·L -1 Cr rat serum for 48 h. Mouse embryonic development in 0.6 mg·L -1 Cr group was similar to that of the control. But all higher concentration groups had a significant decrease in development score, yolk sac diameter, head length, crown rump length and dry weight, the 1.2 mg·L -1 Cr and higher concentrations also resulted in embryotoxicity and abnormalities, which were similar to that of the rat embryo. These results indicate that K 2Cr 2O 7 is developmental toxic in both rats and mice but rats are more sensitive than mice.

关 键 词：重铬酸钾 胚胎发育毒性 

分 类 号：R995[医药卫生—毒理学]