乙肝患者前S1抗原HBV-DNA及血清标志物的关系探讨  

Relations between Pre-S1 antigen with HBV-DNA and serum markers in hepatitis B patients

作　　者：冯新国 欧阳淑兰 

机构地区：[1]株洲市三三一医院检验科,湖南株洲412002

出　　处：《现代医药卫生》2011年第16期2415-2416,共2页Journal of Modern Medicine & Health

摘　　要：目的:探讨乙肝患者前S1抗原与血清标志物和HBV-DNA的关系,及对乙肝的诊疗价值。方法:用酶联免疫法(ELISA)检测283例乙肝血清HBV-M和PreS1-Ag,用荧光定量PCR法同时检测HBV-DNA。结果:对乙肝血清标志物5种模式进行分析,模式①中PreS1-Ag的阳性率(72.9%)和HBV-DNA的阳性率(95.8%)高于模式②中PreS1-Ag的阳性率(37.7%)和HBV-DNA的阳性率(40.6%),两种模式间差异有统计学意义(P<0.01)。128例PreS1-Ag阳性患者中HBV-DNA阳性率78.1%(100/128),HBeAg阳性率为54.7%(70/128),两者差异有统计学意义(P<0.01)。结论:PreS1-Ag与HBV-DNA在反映不同血清型乙肝病毒的复制方面一致性较好;尤其是HBeAg阴性的乙肝患者,检测血清PreS1-Ag和HBV-DNA在乙肝诊断和治疗中具有重要临床意义。Objective：To analyze the relations between Pre-S1 antigen（PreS1-Ag） with serum markers and HBV-DNA in hepatitis B patients,and to investigate their diagnosis and treatment value to hepatitis B.Methods：283 patients with hepatitis B were tested for serum HBV-M and PreS1-Ag by ELISA and HBV-DNA by the fluorescence-assay PCR.Results：The 5 kinds of HBV serum markers mode were analyzed.In mode 1,the positive rates of PreS1-Ag and HBV-DNA were 72.8% and 95.09% respectively.In mode 2,the positive rates of PreS1-Ag and HBV-DNA were 37.7% and 40.6% respectively.The positive rates of PreS1-Ag and HBV-DNA in mode 1 were higher than those in mode 2,showing statistical difference between the two modes（P0.01）.Among 128 cases of positive PreS1-Ag,the positive rates of HBV-DNA and HBeAg were 78.1%（100/128） and 54.7%（70/128） respectively with statistical difference（P0.01）.Conclusion：PreS1-Ag and HBV-DNA have good consistence in reflecting different serotypes of hepatitis B virus replication.Especially in the patients with HBeAg-negative hepatitis B,detecting serum HBV-DNA and PreS1-Ag has great clinical significance in the diagnosis and treatment of hepatitis B.

关 键 词：乙型肝炎病毒DNA 乙型肝炎E抗原 乙型肝炎前S1抗原 乙肝血清模式 

分 类 号：R446[医药卫生—诊断学]