哈维氏弧菌生物被膜体外模型的建立及成膜特性研究  

Establishment of in vitro biofilm model and characteristics of biofilm formation of Vibrio harveyi

作　　者：李海平[1] 鄢庆枇[1] 徐晓津[1] 苏永全[2] 覃映雪[1] 

机构地区：[1]集美大学水产学院福建省高校水产科技与食品安全重点实验室,福建厦门361021 [2]厦门大学海洋与环境学院,福建厦门361005

出　　处：《热带海洋学报》2011年第3期99-104,共6页Journal of Tropical Oceanography

基　　金：国家高技术研究发展计划项目(2007AA09Z115)

摘　　要：采用改良微孔板法建立哈维氏弧菌Vibrio harveyi TS-628菌株生物被膜体外模型,并进一步研究环境因子对哈维氏弧菌体外形成生物被膜的影响。结果发现,哈维氏弧菌TS-628菌株可以在聚苯乙烯板中形成生物被膜,并且该菌生物被膜的形成受起始菌液浓度、温度、pH等理化因子的显著影响。温度为30℃、NaCl质量分数为3%—6%、pH为7、孵育时间为36h是哈维氏弧菌形成生物被膜的最佳条件。此外哈维氏弧菌在分别经表皮黏液、鳃黏液、肠道黏液、肝脏提取液、脾脏提取液、肌肉提取液包被的基质上成膜效果显著,其中在经肝脏提取液包被的基质上形成的生物被膜量显著高于在其他生物基质上形成的生物被膜量(p<0.01)。Modified microtiter-plate test was used to establish an in vitro biofilm model of Vibrio harveyi TS-628 strain.Effect of environmental factors on bacterial biofilm formation in vitro was also studied.The results show that V.harveyi TS-628 strains could form biofilm in polystyrene plates,and biofilm formation of this bacterium was significantly affected by different environmental factors,such as temperature,initial bacterial concentration,pH,NaCl concentration,and so on.The optimum conditions for biofilm formation of V.harveyi TS-628 strain are at temperature of 30℃,pH=7,NaCl concentration of 3%？6%,and using 36h incubation time.Furthermore,V.harveyi TS-628 strain could form biofilm on the walls coated with skin mucus,gill mucus,intestinal mucus,liver extract,spleen extract,and muscle extract.The biofilm formation of V.harveyi on the walls coated with liver extract exhibited a remarkably higher value of D（590）（p0.01）than that on the walls coated with other tissue extracts.

关 键 词：哈维氏弧菌 生物被膜 环境因子 微孔板法 生物基质 

分 类 号：P735.2[天文地球—海洋生物学]