X射线全身照射对小鼠胸腺细胞p53、p21、GADD45及MDM2蛋白表达的影响  

Effect of whole body X-irradiation on the expression of p53,p21, GADD45 and MDM2 proteins in mouse thymocytes

作　　者：鞠桂芝[1] 傅海青[1] 苏旭[1] 付士波[1] 刘树铮[1] 

机构地区：[1]卫生部放射生物实验室,长春130021

出　　处：《白求恩医科大学学报》1999年第6期677-678,共2页Journal of Norman Bethune University of Medical Science

基　　金：国家自然科学基金

摘　　要：目的：观察电离辐射全身照射对小鼠胸腺细胞ｐ５３、ｐ２１、ＧＡＤＤ４５ 及ＭＤＭ２ 蛋白表达的影响。方法：采用间接免疫荧光标记，流式细胞术检测蛋白表达的变化。结果：２ Ｇｙ Ｘ射线照射后１～８ ｈ ｐ５３ 蛋白表达显著增高；照射后４～４８ ｈ ｐ２１ 蛋白表达显著增高；ＭＤＭ２ 蛋白在照后４ ｈ 及８ ｈ 显著增高；而ＧＡＤＤ４５ 蛋白表达在照后１～４８ ｈ 内无明显变化。结论：电离辐射可诱导胸腺细胞ｐ５３、ｐ２１ 及ＭＤＭ２ 蛋白表达增高。此结果将对辐射诱导胸腺细胞Ｇ１ 期阻滞的分子通路研究有重要意义。Objective:To investigate the effect of ionizing radiation on the expression of p53,p21,GADD45 and MDM2 proteins of thymocytes in mice.Methods:Flow cytometry (FCM) was used to analyze protein expression of thymocytes for their immunofluorescence stained with monoclonal antibodies.Results:It was demonstrated that after 2 Gy whole body irradiation with X rays,p53 protein expressions of mouse thymocytes were significantly increased at 1 h to 8 h;p21 at 4 h to 48 h; and MDM2 at 4 h and 8 h.However,no significant change was found in GADD45 protein expression following irradiation.Conclusion:The protein expression of p53,p21 and GADD45 of thymocytes could be induced by ionizing radiation,which might be involved in the molecular pathway of G 1 arrest after exposure.

关 键 词：电离辐射 胸腺细胞 P53 P21 GADD45 MDM2 

分 类 号：Q691.5[生物学—生物物理学]