雌激素诱导皮肤成纤维细胞向肌成纤维细胞表型转分化的机制  

Mechanism of dermal fibroblasts transdifferentiation into myofibroblasts induced by estrogen

作　　者：熊杰[1] 李勤[1] 陈小波[1] 余文林[1] 程飚[1] 

机构地区：[1]广州军区广州总医院整形外科,510010

出　　处：《中华医学美学美容杂志》2011年第4期294-297,共4页Chinese Journal of Medical Aesthetics and Cosmetology

基　　金：广东省自然科学基金资助项目（编号：0700053）

摘　　要：目的探讨雌激素诱导皮肤成纤维细胞向肌成纤维细胞转分化的可能途径和调控机制。方法将人皮肤成纤维细胞分为6组：对照组（A组）、雌激素组（B组）、雌激素＋ICI-182780组（C组）、雌激素＋SB203580组（D组）、雌激素＋PD98059组（E组）和雌激素＋SP600125组（F组）。各组细胞经同步化处理后，直接以雌激素刺激或经上述各激酶抑制剂预处理后再以雌激素刺激。收集细胞，一部分以单细胞逆转录聚合酶链反应（RT-PCR）检测α平滑肌肌动蛋白（alpha smooth muscleactin，（2-SMA）阳性表达百分比，另一部分细胞抽提总RNA后采用实时荧光定量RT—PCR检测α—SMA的表达水平变化。结果B组的α—SMA表达水平和阳性百分比与A组比较显著升高（P〈0．01），而C组和F组与B组比较α—SMA表达水平和阳性百分比升高的作用则被显著抑制（P〈0．05）。结论在雌激素诱导成纤维细胞向肌成纤维细胞的转分化过程中，雌激素G受体及JNK—MAPK信号转导途径起到重要作用。Objective To explore the possible pathway and regulatory mechanism of dermal fibroblasts＇ transdifferentiation into myofibroblasts induced by estrogen. Methods Human dermal fibroblasts were divided into six groups （A： control; B： estrogen; C： estrogen nu ICI-182780; D： estrogen ＋ SB203580; E.. estrogen ＋ PD98059; F： estrogen ＋ SP600125）. The cells were collected for RNA extraction and the expression of α-SMA was detected by real time quantitative RT-PCR. Some cells were analyzed by single cell RT-PCR to detect positive expression percentage of α-SMA. Results The expression and positive rate of α-SMA in estrogen group were significantly increased （Group B vs. Group A, 7.385±0.246 vs 1. 367±0. 034, P〈0.01） and those in ICI-182780 group and SP600125 group were significantly inhibited （Groups C and F vs. Group B, 4. 619 ± 0. 164, 2. 409±0. 091 vs 7. 385±0. 246, P（0.05）. Conclusions In the process of fibroblast transdifferenti ation into myofibroblasts induced by estrogen, estrogen β receptor and JNK-MAPK signal transduction pathway may play an important role.

关 键 词：雌激素 雌激素B受体 成纤维细胞 转分化 激酶抑制剂 

分 类 号：R96[医药卫生—药理学]