N-甲基-N-顺式-苯乙烯基-桂皮酰胺对青霉素诱发惊厥大鼠神经细胞内钙离子含量的影响  

Effects of N-methyl-N-cis-styryl-cinnamamide on intracellular calcium ion concentration(cell[Ca^(2-)] i) in seizure rats induced by penicillin

作　　者：邬继栋[1,2] 柳国胜[3] 高江晖[1] 沈伟哉[1] 

机构地区：[1]暨南大学医学院人体解剖学教研室,广州510632 [2]朔州市第一人民医院,朔州036002 [3]暨南大学附属第一医院儿科,广州510632

出　　处：《中药药理与临床》2011年第3期16-20,共5页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金(30471216);广东省自然科学基金团队项目(039213)

摘　　要：目的:检测黄皮核提取物N-甲基-N-顺式-苯乙烯基-桂皮酰胺(N-methyl-N-cis-styryl-cinnamamide,s-MSC)对皮层定位注射青霉素(Penicillin,PNC)诱发惊厥大鼠脑神经细胞内钙离子含量的影响,初步探讨其抗惊机理。方法:20只大鼠分为5组:正常对照组(20 ml/L吐温80+生理盐水)、模型对照组(20 ml/L吐温80+PNC)、丙戊酸钠120 mg/kg+PNC7、5 mg/kg s-MSC组(s-MSC 75 mg/kg+PNC)、150 mg/kg s-MSC组(s-MSC 150 mg/kg+PNC)。建立皮层定位注射PNC诱发大鼠癫痫模型,流式细胞术检测各组大鼠左侧大脑初级、次级躯体运动皮层及左侧海马神经细胞内钙离子含量。结果:皮层定位注射PNC后大鼠脑神经元内钙离子含量增加(P<0.01),丙戊酸钠及150 mg/kg的s-MSC均可使致癫大鼠脑神经元内钙离子含量降低(P<0.01)7,5 mg/kg s-MSC对于细胞内钙离子的降低无统计学意义。结论:黄皮核提取物N-甲基-N-顺式-苯乙烯基-桂皮酰胺对抗大鼠皮层定位注射PNC诱发的惊厥可能与其降低神经元内钙离子含量有关。To investigate the effect of the seed extract of Clausena lansium,N-methyl-N-cis-styryl-cinnamamide,on intracellular calcium ion concentration（cell[Ca2＋] i） in seizure rats induced by penicillin and explore its mechanism of anticonvulsant action.Method：Twenty Sprague-Dawely rats were randomly divided into five groups with 4 rats in each group：normal control（20 ml/L Tween 80 ＋ Normal Saline）,model control（20 ml/L Tween 80 ＋ Penicillin）,positive control（Sodium Valproate 120 mg/kg ＋ Penicillin）,low dose treatment（s-MSC 75 mg/kg ＋ Penicillin）,high dose treatment（s-MSC 150 mg/kg ＋ Penicillin）.The seizure rat model was established by penicillin localized injected in cortex.The change of intracellular calcium ion concentration（cell[Ca2＋] i） in rats neuron of the left side of the primary,secondary somatic motor cortex and left hippocampus was detected by flow cytometry.Result：The neuronal intracellular calcium ion concentration（cell[Ca2＋）] i） of rat cerebral cortex was significantly increased after penicillin injection,compared with the normal control group（p〈0.01）.Compared with the model group,high dose N-methyl-N-cis-styryl-cinnamamide and Sodium Valproate can significantly decreased intracellular calcium ion concentration（cell[Ca2＋] i）（P〈0.01）.However,there was no significant difference in intracellular calcium concentration（cell[Ca2＋] i） between the model and low dose cinnamamide treatment groups.Conclusion：The anticonvulsive function of the seed extract of Clausena lansium,N-methyl-N-cis-styryl-cinnamamide,might relate with decreasing intracellular calcium ion concentration（cell[Ca2＋] i） level.

关 键 词：N-甲基-N-顺式-苯乙烯基-桂皮酰胺 细胞内钙离子 青霉素诱发 

分 类 号：R285[医药卫生—中药学]