去乙酰紫玉盘素通过抑制ERK/MAPK通路抗肝癌增殖  

Desacetyluvaricin resists proliferation of hepatoma cells through inhibiting ERK/MAPK pathway

作　　者：任凤梅[1] 舒秉俊[1] 罗时辉[1] 王鹰[1] 章跃平[1] 徐安[1] 

机构地区：[1]南昌市第二医院,南昌330003

出　　处：《中药药理与临床》2011年第3期28-30,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：江西省卫生厅中医药科研基金课题(课题编号:2009A075)

摘　　要：目的:观察番荔枝内酯去乙酰紫玉盘素(Desacetyluvaricin)对人肝癌细胞HepG2中细胞外信号调节激酶(extracellular signal regulated kinase,ERKs)表达水平的影响,探讨去乙酰紫玉盘素对ERK/MAPK信号传导通路的影响及其抗肝癌增殖的机制。方法:以12.52、5、50μg/ml的Desacetyluvaricin处理HepG2细胞,Western Blot检测ERK、p-ERK蛋白表达水平的变化,分析去乙酰紫玉盘素对ERK/MAPK信号通路的影响。结果:Desacetyluvaricin处理HepG2细胞后,ERK和p-ERK蛋白表达水平明显下降,并呈剂量依赖关系,其中Desacetyluvaricin以50μg/ml的浓度处理HepG2细胞时免疫印迹条带的发光强度最弱,ERK和p-ERK蛋白的表达水平最低。结论:去乙酰紫玉盘素抗肝癌增殖可能与其抑制ERK/MAPK通路有关。Objective：To observe the effect of desacetyluvaricin on extracellular signal regulated kinase（ERKs） expression in hepatoma carcinoma HepG2 cells,and explore the mechanisms of the antitumor effects of desacetyluvaricin.Methods：HepG2 cells were treated with desacetyluvaricin,Western Blot was performed to examine the expressions of ERK and p-ERK in the cells.Results ：The expression of ERK and p-ERK were significantly lowered in the cells after treatment.Conclusion ：desacetyluvaricin can resist proliferation of hepatoma cells,and the possible mechanisms may be related to the inhibition of ERK/MAPK pathway in HepG2 cells.

关 键 词：番荔枝内酯 ERK/MAPK 肝细胞癌(HCC) 

分 类 号：R285[医药卫生—中药学]