淡豆豉异黄酮对AngⅡ诱导大鼠血管平滑肌细胞周期的影响  

Effect of semen sojae preparatum isoflavone on rat vascular smooth muscle cell cycle induced by angiotensin Ⅱ

作　　者：任艳青[1] 李清[1] 刘姣[1] 曹秀莲[1] 王鑫国[1] 

机构地区：[1]河北医科大学中医学院,河北省实验动物重点实验室,石家庄050091

出　　处：《中药药理与临床》2011年第3期44-47,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：河北省自然科学基金资助项目(No.C2009001061)

摘　　要：目的:观察淡豆豉异黄酮对血管紧张素Ⅱ(Angiotensin Ⅱ,Ang Ⅱ)诱导大鼠血管平滑肌细胞(Vascular Smooth MuscleCell,VSMC)细胞周期的影响,探讨其抑制增殖的可能机制。方法:原代培养大鼠VSMC,建立AngⅡ诱导VSMC增殖模型,MTT法观察淡豆豉异黄酮对Ang Ⅱ诱导VSMC增殖的干预作用,流式细胞仪观察淡豆豉异黄酮干预细胞后细胞周期的变化。结果:淡豆豉异黄酮50μg/L预孵育12、24 h,100μg/L预孵育1、4、12、24 h,200μg/L预孵育1、4、24 h,500μg/L预孵育4 h可显著抑制AngⅡ诱导的VSMC增殖;50、100、200μg/L作用24 h抑制增殖作用最为明显,并可显著增加VSMC G0/G1期比例,降低S期比例。结论:淡豆豉异黄酮抑制Ang Ⅱ诱导的VSMC增殖作用机制可能与阻止细胞由G0/G1期进入S期,进行VSMC细胞周期的调控有关。Objectives：To study the effect of semen sojae preparatum isoflavone（SSPI） on rat vascular smooth muscle cell（VSMC） cycle induced by Angiotensin Ⅱ（AngⅡ） and to explore the mechanism of inhibiting proliferation.Methods：VSMC of rat were primarily cultured,a cellproliferating model of VSMC induced by AngⅡ was established.The effect of SSPI on VSMC proliferation induced by Ang Ⅱ was analyzed by MTT method,the change of VSMC cell cycle was observed by flow cytometry.Results：SSPI of 50μg/L preincubated 12,24 h,100μg/L preincubated 1,4,12,24 h,200μg/L preincubated 1,4,24h,500μg/L preincubated 4h obviously inhibited the proliferation of VSMC induced by AngⅡ;SSPI of 50,100,200 μg/L that effected 24h most inhibited VSMC proliferation,the cell numbers in G0/G1 phase obviously increased and those of S phase decreased significantly.Conclusions：The proliferation of VSMC induced by AngⅡ can be inhibited by SSPI.The mechanism might be related to preventing VSMC from G0/G1 phase to S phase,and regulating VSMC cell cycle.

关 键 词：淡豆豉异黄酮 动脉粥样硬化 血管平滑肌细胞 增殖 AngⅡ 细胞周期 

分 类 号：R285[医药卫生—中药学]