复方红景天制剂对高+Gz应激大鼠心肌线粒体超微结构的影响  

Effect of rhodiola crenulata compounds on ultrastructure injury of myocardial mitochondria in rats exposured to high +Gz stress

作　　者：陈良恩[1] 王永新[1] 魏日胞[2] 詹皓[1] 辛益妹[1] 

机构地区：[1]空军航空医学研究所第一研究室,北京100142 [2]解放军总医院肾病科,北京100853

出　　处：《中华老年多器官疾病杂志》2011年第3期261-264,共4页Chinese Journal of Multiple Organ Diseases in the Elderly

基　　金：全军和空军医药卫生基金(06MA265;KW08Z06);国家自然科学基金(81073083)~~

摘　　要：目的观察复方红景天制剂对高+Gz应激大鼠心肌线粒体超微结构的影响。方法将60只SD大鼠随机分为空白对照组、应激对照组、高+Gz应激组、复方高剂量组、复方中剂量组和复方低剂量组,每组10只。各组动物均灌胃给药(20ml/kg),每日一次,连续14 d。复方高剂量组、中剂量组和低剂量组分别按3.0,1.5和0.75g/kg给予复方红景天制剂,空白对照组、应激对照组和高+Gz应激组给予等量生理盐水。第15天进行离心机暴露。观察各组动物心肌线粒体超微结构并计算线粒体的体视学参数。结果高+Gz应激组肌原纤维离散,线粒体肿胀变性,线粒体基质电子密度降低,线粒体嵴减少、断裂或消失。复方红景天制剂预处理组线粒体超微结构损伤减轻,其中高剂量组线粒体超微结构与对照组接近。高+Gz应激组线粒体体视学参数与空白对照组和应激对照组相比,体积密度、表面积密度、平均体积、平均表面积变大,数密度变小。与高+Gz应激组相比,复方红景天制剂预处理组线粒体体积密度、表面积密度、平均体积、平均表面积变小、数密度变大;中剂量组和高剂量组大部分指标与对照组相比没有明显差异。结论复方红景天制剂对高+Gz应激导致的大鼠心肌线粒体超微结构损伤具有一定的保护作用。Objective To study the effect of rhodiola crenulata compounds on ultrastructural injury of myocardial mitochondria in rats after high ＋Gz stress.Methods Sixty healthy SD rats were divided randomly into six groups,including blank control group, stress control group,high ＋Gz stress group,high dose compounds group,medium dose compounds group and low dose compounds group,with 10 in each group.Menstruum（20 ml/kg） was fed to each rat once per day for 14 days.High dose compounds group,medium dose compounds group and low dose compounds group were fed with rhodiola crenulata compounds at doses of 3.0,1.5,and 0.75 g/kg respectively.Blank control group,stress control group and high ＋Gz stress group were fed with equal volume saline.Ultrastructural changes of myocardial mitochondria were observed under electron microscope and stereology parameters were calculated. Results The ultrastructural changes of the cardiac muscle were observed in rats of high ＋Gz stress group：myofibril disorganized; mitochondria swelled,degenerated and decreased;mitochondrial cristae broke or disappeared.The injury of mitochondria ultrastructure induced by high ＋GZ stress relieved in rhodiola crenulata compounds preconditioning groups and that of high dose compounds group was essentially normal.Compared with blank control group or stress control group,the stereology parameters of high ＋Gz stress group,including volume density,surface density,average volume and average surface area were significantly higher,and numerical density was lower.However,these parameters in rhodiola crenulata compounds preconditioning groups changed reversely. Most parameters of high dose compounds and medium dose compounds groups had no significant difference with those of blank or stress control groups.Conclusion Rhodiola crenulata compounds has some protective effects on injury of myocardial mitochondria ultrastructure in rats exposed to high ＋GZ stress.

关 键 词：复方红景天制剂 高+Gz应激 线粒体 超微结构 

分 类 号：R542.2[医药卫生—心血管疾病]