血管紧张素Ⅱ预处理提高间充质干细胞耐缺氧能力  被引量:3

AngiotensinⅡ preconditioning prevents mesenchymal stem cells from hypoxic injury

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作  者:高景红[1] 孙传波[1] 张竞文[1] 胡亮[1] 李庆平[1] 

机构地区:[1]南京医科大学药理学暴,江苏南京210029

出  处:《南京医科大学学报(自然科学版)》2011年第8期1092-1096,共5页Journal of Nanjing Medical University(Natural Sciences)

基  金:国家自然科学基金资助(30973534)

摘  要:目的:探讨血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)预处理对骨髓间充质干细胞(mesenchymal stem cells,MSCs)耐缺氧能力的影响。方法:分离培养大鼠MSCs,应用CD29、CD11b/c抗体进行细胞鉴定。对细胞以不同浓度AngⅡ预处理3 h,采用缺氧联合无血清(hypoxia/SD)的方法诱导缺氧损伤模型;应用血管紧张素1型受体(AT1)拮抗剂氯沙坦(losartan)、血管紧张素2型受体(AT2)拮抗剂PD123319进行干预。应用MTT和CCK-8法测定细胞活力以确定最佳刺激浓度,采用台盼蓝染色,CCK-8检测和流式细胞术测定各组细胞活力和凋亡率。结果:①MSCs表面抗原CD29阳性率>97%,CD11b/c阳性率<1%;②AngⅡ预处理显著提高MSCs的活力,减少细胞凋亡率;③AngⅡ对MSCs的预适应保护作用呈现一定的浓度依赖性,最佳浓度为10nmol/L;④氯沙坦可抑制AngⅡ的预适应细胞保护作用,而PD123319无此效应。结论:AngⅡ通过AT1受体发挥对MSCs的预适应保护作用。Objective:To investigate whether Angiotensin II protects mesenchymal stem cells (MSCs) from hypoxic injury. Methods:The MSCs were isolated and cultured,and their purities were identified with the spindle-fibroblastic morphology characterizahon by microphotograph,and the phenotypes were tested by flow cytometry with anti-CD29 and anti-CD1 lb/c antibodies. Before preconditioned by Ang II, the MSCs were treated with or without angiotensin 1 (AT1) receptor antagonist losartan, angiotensin 2 (AT2) receptor antagonist PD123319. Then the cells were suffered from hypoxia and serum deprivation for 24 hours. The cell viability and apoptosis were determined by trypan blue staining, MTY, CCK-8 assay and Annexin V-FITC staining. Results: (1) MSCs expressed CD29,but not the CD1 lb/c. The purity of MSCs employed in this study was up to 97%; (2) Ang II preconditioning (PC) markedly protected MSCs from hypoxic injury. However,these effects were abohshed by prior treatment with losartan rather than PD123319. Conclusion:These results suggest that Ang II preconditioning protects MSCs from hypoxic injury through AT1 receptor.

关 键 词:骨髓间充质干细胞 血管紧张素Ⅱ 预处理 缺氧 

分 类 号:R329.2[医药卫生—人体解剖和组织胚胎学]

 

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