两种测定大鼠心肌缺血再灌注损伤后梗死面积方法的比较  

Comparison of the two different methods of measuring infarct size after rats were subjected to ischemia and reperfusion

作　　者：王卓璎[1] 张雯[2] 李晓珍[2] 韩艺[3] 陈燕萍[1] 鲁翔[1] 季勇[2] 

机构地区：[1]南京医科大学第二附属医院老年科,江苏南京210011 [2]南京医科大学动脉粥样硬化中心,江苏南京210029 [3]南京医科大学第一附属医院老年科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2011年第8期1101-1105,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金(81070275);国家基础研究项目973(2010CB535010)

摘　　要：目的:探索一种在体测定大鼠心肌缺血再灌注损伤后心肌梗死面积的方法,并与传统的离体染色方法进行了比较,确定其可行性。方法:结扎雄性成年Sprague-Dawley大鼠冠状动脉前降支,给予缺血30 min,再灌注6 h,用超声心动图评价大鼠的心功能,用生化分析仪测定血清中的肌酸激酶(creatine kinase,CK)活性。再灌注24 h后,用离体和在体两种方法进行TTC(triphenyltetrazolium chloride,2,3,5-氯化三苯基四氮唑)-Evans blue双染,比较两种方法的成功率、心梗面积/缺血区面积(in-farct area/risk area,IA/RA)与血清CK活性的相关性。结果:在体染色法的IA/RA与离体染色法没有显著性差异,但成功率高。其IA/RA与血清CK活性的相关性高于离体染色法。结论:在体染色法与离体染色法相比,操作简易,能更准确地反映心肌缺血再灌注损伤的程度。Objective：To develop a new method of measuring infarct size by staining hearts in vivo after ischemia-reperfusion,and compare with the traditional method of staining in vitro to determine its feasibility.Methods：Adult male Sprague-Dawley rats were subjected to 30 min myocardial ischemia and 6 h reperfusion by ligation of the left anterior descending coronary artery.Cardiac function was evaluated by echocardiography and serum creatine kinase（CK） activity was also measured by a biochemical analyzer.After reperfusion for 24 h,hearts were staining by triphenyltetrazolium chloride（TTC）-Evans blue in vitro or in vivo.Infarct area（IA） / risk area（RA） was calculated.Finally we compared the success rate of the two methods,and made correlation analysis of the IA / RA with serum CK activity.Results：There was no significant difference in IA / RA staining by the two different methods.However,success rate of staining in vivo was significant higher than that of staining in vitro.Besides,compared with staining in vitro,there was a stronger correlation between IA / RA and CK activity by staining in vivo.Conclusion：The method of staining in vivo is much easier to operate,and it can evaluate the damage by myocardial ischemia-reperfusion injury more accurately than that of staining in vitro.

关 键 词：心肌缺血再灌注损伤 梗死面积 Trc-Evans blue染色 

分 类 号：R318.11[医药卫生—生物医学工程]