2,2’,4,4’-四溴联苯醚对人神经母细胞瘤SH-SY5Y细胞胞内游离钙离子浓度的影响  被引量:1

Effect of 2,2',4,4'-tetrabromodiphenyl Ethers on Intracellular Free Calcium Concentration in Human Neuroblastoma SH-SY5Y Cells

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作  者:陈义虎[1] 姜春阳[1] 吴雪[1] 张舜[1] 张小非[1] 夏涛[1] 王爱国[1] 

机构地区:[1]华中科技大学同济医学院公共卫生学院劳动卫生与环境卫生学系环境与健康教育部重点实验室,湖北武汉430030

出  处:《环境与健康杂志》2011年第8期659-662,共4页Journal of Environment and Health

基  金:国家自然科学基金面上项目(81072266)

摘  要:目的研究22,’,4,4’-四溴联苯醚(2,2’,4,4’-tetrabromodiphenyl ethers,PBDE-47)对人神经母细胞瘤SH-SY5Y细胞胞内游离钙离子([Ca2+]i)浓度的影响。方法取对数生长期的SH-SY5Y细胞,分别染毒终浓度为0(溶剂对照)、1、5、10μmol/L的PBDE-47溶液。采用MTT法检测PBDE-47体外染毒24 h后SH-SY5Y细胞存活率。利用钙离子荧光探针Fluo-3/AM标记体外培养的SH-SY5Y细胞,在激光扫描共聚焦显微镜下持续动态观测1 h内胞内钙离子荧光强度变化。采用流式细胞仪检测PBDE-47染毒3、6、12、24 h后[Ca2+]i变化。结果与溶剂对照组比较,5、10μmol/L PBDE-47染毒组细胞存活率明显下降(P<0.05);不同浓度PBDE-47染毒1 h后[Ca2+]i浓度均高于染毒0 h(P<0.05);10μmol/LPBDE-47染毒3、6、12、24 h后[Ca2+]i浓度均升高(P<0.05),5μmol/L PBDE-47染毒3、6、24 h后[Ca2+]i浓度也升高(P<0.05),而1μmol/L PBDE-47仅染毒6 h后[Ca2+]i浓度升高(P<0.05)。结论 PBDE-47染毒可使SH-SY5Y细胞[Ca2+]i浓度发生早期且持续升高,提示PBDE-47导致的SH-SY5Y细胞损伤可能与细胞内钙离子稳态改变有关。Objective To investigate the effect of 2,2' ,4,4' -tetrabromodiphenyl ethers (PBDE-47) on intracellular free calcium ([Ca^2+]i) concentration in human neuroblastoma SH-SY5Y cells. Methods Exponentially growing cells were treated with PBDE-47 at the doses of 0 (solvent control), 1,5 and 10 μmol/L respectively. MTF method was used to detect the cell viability in SH-SY5Y cells treated for 24 h. Fluo-3/AM, a fluorescent calcium indicator, was used to monitor changes of the intracellular calcium signaling, under the laser scanning confocal microscope the [Ca^2+]; level was consecutively detected for 1 h. After 3,6,12 and 24 h of treatment, the levels of [Ca^2+]; were measured respectively by using flow cytometry. Results Compared with the solvent control,the cell viabilities significantly decreased in the cells treated by 5,10 μmol/L PBDE-47 (P〈0.05). In the cells treated by different concentrations of PBDE-47 for 1 h, the level of [Ca^2+]; was higher than that in 0 h group (P〈0.05). Furthermore,after exposure to 10 μmol/L PBDE-47 for 3,6,12 and 24 h, the levels of [Ca^2+]; increased significandy (P〈0.05) and after exposure to 5 μmol/L PBDE-47 for 3,6 and 24 h, the levels of [Ca^2+]; significantly increased also (P〈0.05), but only at 6 h after exposure to 1 μmol/L PBDE-47,the level of [Ca^2+]; increased significantly (P〈0.05). Conclusion PBDE-47 can induce the level of [Ca^2+]; increase at the early stage in SH-SY5Y cells,and the elevation of [Ca^2+]; is long-lasting,which indicates that calcium homeostasis may be related to the SH-SY5Y cells damage induced by PBDE-47.

关 键 词:2 2’ 4 4’-四溴联苯醚 入神经母细胞瘤SH-SY5Y细胞 胞内游离钙离子浓度 

分 类 号:R994.6[医药卫生—毒理学]

 

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