氟、钙与碘联合染毒对大鼠甲状腺系数和形态结构的交互作用  

Interaction of Fluorine,Calcium and Iodine on Thyroid Index and Morphosis in Rats

作　　者：李宏彬[1] 申旭波[2] 许洁[2] 董国宾[2] 邹焰[2] 

机构地区：[1]新乡医学院流行病与卫生统计学教研室,河南新乡453003 [2]遵义医学院预防医学教研室

出　　处：《环境与健康杂志》2011年第8期674-676,F0003,共4页Journal of Environment and Health

基　　金：贵州省教育厅自然科学类资助项目(黔教科20022321);遵义医学院优秀青年基金(G-005)

摘　　要：目的观察氟、钙和碘联合染毒对甲状腺系数和形态结构的交互作用。方法应用2×2×2析因实验设计将80只Wistar大鼠分成8组,分别为适中碘(3.5μg/d)+适中氟(90μg/d)+适中钙(13 mg/d)染毒组、适中碘+适中氟+高钙(260mg/d)染毒组、适中碘+高氟(2 700μg/d)+适中钙染毒组、适中碘+高氟+高钙染毒组、低碘(0.23μg/d)+适中氟+适中钙染毒组、低碘+适中氟+高钙染毒组、低碘+高氟+适中钙染毒组、低碘+高氟+高钙染毒组,每组10只,雌雄各半。喂养6个月后,观察甲状腺系数和形态结构的变化。结果低碘组雄性大鼠甲状腺系数高于适中碘组(F=6.234,P=0.018)。氟与钙联合染毒对雌性大鼠甲状腺系数有交互作用(F=4.483,P=0.042;),适中碘时显示协同作用,低碘时显示拮抗作用;氟与碘联合染毒对雌性大鼠甲状腺系数有拮抗作用(F=4.358,P=0.045)。固定为适中氟和适中钙时,低碘组雌性大鼠的甲状腺系数高于适中碘组(t=4.089,P=0.003);固定为适中氟和高钙时,低碘组雌性大鼠的甲状腺系数高于适中碘组(t=2.716,P=0.026);固定为适中碘和适中钙时,高氟组雌性大鼠的甲状腺系数低于适中氟组(t=0.016,P=3.047);固定为适中碘和高氟时,高钙组雌性大鼠的甲状腺系数高于适中钙组(t=3.982,P=0.004)。高氟组雌性大鼠滤泡壁上皮细胞长度低于适中氟组(F=6.196,P=0.018),高氟组雄性大鼠光密度低于适中氟组(F=4.550,P=0.041),高氟组雄性大鼠黑度高于适中氟组(F=7.744,P=0.009)。其他各组间无差异(均P>0.05)。结论低碘可引起雄性和雌性大鼠甲状腺系数的增加;氟可引起大鼠甲状腺系数降低、甲状腺细胞增加、胶质减少。Objective To study the interaction of fluorine,calcium and iodine on thyroid weight and morphological structure in rats. Methods Eight Wistar rats were randomly divided into 8 groups： moderate concentrations of fluorine（90 μg/d）, calcium （ 13 mg/d） and iodine （3.5 μg/d）; moderate concentrations of fluorine ,iodine and higher concentration of calcium（260 mg/d）; moderate concentrations of calcium, iodine and higher concentration of fluorine （2 700 μg/d）; moderate concentration of iodine and higher concentrations of fluorine ,calcium; moderate concentrations of fluorine ,calcium and lower concentration of iodine （0.23 μg/d）; moderate concentration of fluorine,higher concentration of calcium and lower concentration of iodine; moderate concentration of calcium,higher concentration of fluorine and lower concentration of iodine; higher concentrations of fluorine, calcium and lower concentration of iodine based on 2x2x2 factorial design. After six months of treatment,thyroid index was measured and pathological examination was conducted. Results Thyroid weights of male rats in lower iodine group was higher than that in moderate iodine group. Significant interaction effects of fluorine and calcium, fluorine and iodine on thyroid index of female rats were found. Significant interaction effects of fluorine and calcium on thyroid coefficient in female rats were found, and the synergy in moderate iodine, antagonism in lower iodine was seen. The interaction effects of combined fluorine with iodine showed an antagonism in female rat thyroid. The pathological examination showed a similar result to that in thyroid index calculation. Conclusion Low iodine intake may increase thyroid index in male and female rats, high fluorine exposure may decrease thyroid index, increase thyroid cell and decrease thyroid colloid.

关 键 词：氟 钙 碘 甲状腺 交互作用 

分 类 号：R994.6[医药卫生—毒理学]