久效磷致大鼠皮肤成纤维细胞凋亡及氧化损伤的作用  

Effect of Apoptosis and Oxidative Damage in Rat Skin Fibroblast Cells Induced by Monocrotophos

作　　者：周亚莉[1] 闫建国[2] 王艳华[1] 张莉莉[1] 何群力[1] 

机构地区：[1]新乡医学院医学微生物学教研室,河南新乡453003 [2]新乡医学院人体解剖学教研室

出　　处：《环境与健康杂志》2011年第8期689-691,共3页Journal of Environment and Health

基　　金：新乡医学院高学历人才科研启动项目

摘　　要：目的研究久效磷对体外培养的大鼠皮肤成纤维细胞凋亡及氧化损伤作用的影响。方法取4只3日龄清洁级SD大鼠的背部皮肤,以组织块法培养大鼠皮肤成纤维细胞,正常传代。取第4代成纤维细胞,调整细胞密度为1.0×106/瓶,当细胞至亚融合状态,分别加入0(对照)、0.01、0.1、1μg/L的久效磷溶液培养6、12、24 h。采用流式细胞术检测大鼠皮肤成纤维细胞凋亡率;采用分光光度比色法检测皮肤成纤维细胞超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活力和丙二醛(MDA)含量。结果 0.1～1μg/L久效磷染毒6、12、24 h时,大鼠皮肤成纤维细胞SOD、CAT活性均低于对照组(P<0.05或P<0.01),仅0.01μg/L久效磷染毒24 h时SOD活力低于对照组(P<0.05)。各浓度久效磷染毒大鼠皮肤成纤维细胞凋亡率和MDA含量均明显高于对照组(P<0.05或P<0.01)。随着久效磷染毒剂量的升高和染毒时间的延长,大鼠皮肤成纤维细胞SOD、CAT活力均呈下降趋势,而MDA含量和细胞凋亡率呈上升趋势。结论久效磷可诱导大鼠皮肤成纤维细胞氧化损伤和细胞凋亡率增高。Objective To investigate the effects of different concentrations of monocrotophos on apoptosis and oxidative damage of rat skin fibroblast cells cultured in vitro. Methods Rat skin fibroblast cells of P4 in cultured were treated with monocrotophos at the doses of 0,0.01,0.1 and 1 μg/L for 6,12,24 h. The cell apoptosis was detected by flow cytometry,and the total activities of SOD, CAT and the contents of MDA in cells were detected by spectrophotometry. Results Treated with the 0.1 and 1 μg/L of monocrotophos for 6,12,24 h in culture media,the total activity of SOD and CAT significantly decreased compared with the controls（P〈0.05 or P〈0.01 ）. Treated with 0.01 μg/L monocrotophos for 6,12,24 h in culture media,only the total activity of SOD for 24 h significantly decreased （P〈0.05）. With the increase of monocrotophos concentration in culture media,the apoptosis rates and the contents MDA of skin fibroblast cells increased significantly compared with the controls （P〈 0.05 or P〈0.01 ）. Conclusion Monocrotophos can induce oxidative damage and promote apoptosis in rat skin fibroblasts.

关 键 词：久效磷 大鼠 皮肤成纤维细胞 氧化损伤 细胞凋亡 

分 类 号：R994.6[医药卫生—毒理学]