Quil A对O型口蹄疫疫苗的免疫增强作用  

Quil A as an Immunoenhancer of Foot-and-Mouth Disease Serotype O Vaccine

作　　者：刘燕瑜[1,2] 任静强[1,2] 张丹[1,2] 谭磊[1,2] 刘存霞[1,2] 靖杰[1,2] 刘昊[1,2] 鲁会军[1] 金扩世[1] 金宁一[1] 

机构地区：[1]军事医学科学院军事兽医研究所吉林省人兽共患病预防与控制重点实验室,长春130062 [2]吉林大学畜牧兽医学院,长春130062

出　　处：《中国生物制品学杂志》2011年第8期907-910,共4页Chinese Journal of Biologicals

基　　金：国家"863"计划资助项目(2006AA10A205);吉林省科技发展计划重点资助项目(20090235)

摘　　要：目的探讨Quil A对口蹄疫(Foot-and-mouth disease,FMD)疫苗的免疫增强作用。方法将O型FMDV抗体阴性的仔猪随机分为4组:1组首次免疫O型口蹄疫核酸苗pVIRIL18P1,第2次免疫重组鸡痘苗vUTAL3CP1;2组首次免疫Quil A+O型口蹄疫核酸苗pVIRIL18P1,第2次免疫重组鸡痘苗vUTAL3CP1;3组两次均免疫猪O型口蹄疫合成肽疫苗;4组两次均免疫PBS。首次免疫后0、14、42 d,检测猪血清中FMDV VP1结构蛋白抗体及细胞因子IL-2、IL-4、IL-10和IFNγ的水平;首次免疫后42 d,检测T淋巴细胞增殖水平。结果 2次免疫后,2组猪血清中FMDV VP1结构蛋白抗体水平、细胞因子IL-2、IL-4、IL-10和IFNγ水平及T淋巴细胞增殖率均显著升高。首次免疫后42 d,2组上述指标中除IL-2水平外,与3组和4组比较差异均有统计学意义(P<0.05或<0.01);除IL-2、IL-10和IFNγ水平外,与1组比较差异均有统计学意义(P<0.05或<0.01)。结论 Quil A对O型口蹄疫疫苗具有较好的免疫增强作用。Objective To investigate the effect of Quil A as an immunoenhancer of foot-and-mouth disease（FMD） serotype O vaccine.Methods Piglets negative for antibody against FMD virus（FMDV） serotype O were randomly divided into four groups.The piglets in group 1 were primed with FMD serotype O DNA vaccine pVIRIL18P1 and boosted with recombinant fowlpox virus FMD vaccine,while those in group 2 were primed with Quil A ＋ FMD serotype O DNA vaccine pVIRIL18P1 and boosted with recombinant fowlpox virus FMD vaccine.However,the piglets in group 3 were immunized twice with FMD serotype O synthetic peptide vaccine,while those in group 4 twice with PBS.The levels of FMDV VP1 structural protein antibody as well as IL-2,IL-4,IL-10 and IFNγ in sera of piglets were determined 0,14 and 42 d,and T lymphocyte proliferation level 42 d after the first immunization.Results After the second immunization,the FMDV VP1 structural protein antibody,IL-2,IL-4,IL-10 and IFNγ levels in sera as well as T lymphocyte proliferation level of piglets in group 2 increased significantly.Forty-two days after the first immunization,the above-mentioned indexes in group 2,except the IL-2 level,showed significant difference with those in groups 3 and 4（P〈0.05 or P〈0.01）,and the indexes except IL-2,IL-10 and IFNγ levels showed significant difference with those in group 1（P〈0.05 or P〈0.01）.Conclusion Quil A showed satisfactory immunopotentiation to FMD serotype O vaccine.

关 键 词：QuilA 佐剂 免疫 口蹄疫疫苗 

分 类 号：S850.651[农业科学—兽医学]