白喉类毒素ELISA检测方法的建立及初步应用  

Development and Preliminary Application of ELISA Method for Diphtheria Toxoid

作　　者：刘朝阳[1] 景辉[1] 陈艳红[1] 张斌[1] 马相虎[1] 朱金华[1] 

机构地区：[1]上海生物制品研究所疫苗一室,上海200052

出　　处：《中国生物制品学杂志》2011年第8期977-979,共3页Chinese Journal of Biologicals

摘　　要：目的建立白喉类毒素双抗体夹心ELISA检测方法,并进行验证及初步应用。方法以马抗白喉类毒素血清作为包被抗体,HRP标记的白喉絮状反应抗毒素作为酶标抗体,建立白喉类毒素双抗体夹心ELISA检测方法,并对该方法进行重复性、特异性验证、最佳线性范围和检测限确定及初步应用。结果经验证,该方法重复性好,特异性强,白喉类毒素含量在0.976 5～125.000 0 ng/ml之间时线性关系良好,检测限为7.812 ng/ml,该方法检测了3批白喉类毒素原液,与动物实验结果基本相符。结论已建立了白喉类毒素双抗体夹心ELISA检测方法,可用于检测白喉类毒素的含量和抗原性。Objective To develop,verify and preliminarily apply a double antibody sandwich ELISA method for diphtheria toxoid.Methods A double antibody sandwich ELISA method was developed using horse antiserum against diphtheria toxoid as coating antibody and HRP-labeled diphtheria flocculation antitoxin as enzyme labeled antibody,then verified for reproducibility and specificity,determined for optimal linear range and detection limit,and applied preliminarily.Results The developed method showed high reproducibility and specificity,of which the optimal linear range and detection limit were 0.976 5 ～ 125.000 0 ng/ml and 7.812 ng/ml respectively.Three batches of bulk diphtheria toxoid were detected by the developed method,and the results were basically consistent with thoes by animal test.Conclusion A double antibody sandwich ELISA method was developed,which might be used for determination of content and antigenicity of diphtheria toxoid.

关 键 词：白喉类毒素 酶联免疫吸附测定 

分 类 号：R378.71[医药卫生—病原生物学] R392.33[医药卫生—基础医学]