TLR4信号通路参与有氧运动对ApoE基因敲除小鼠血管炎性损伤的修复  

TLR4 Signaling Pathway Involved in Repairing of Vascular Inflammation Damaged by Aerobic Exercise on ApoE Knock Out in Mice

作　　者：陈嘉勤[1] 柳维林[1] 张川湘[1] 王茂材[1] 李林[1] 

机构地区：[1]湖南师范大学体育学院,湖南长沙410081

出　　处：《体育科学》2011年第8期53-58,共6页China Sport Science

摘　　要：目的:通过中小强度有氧运动和辛伐他汀药物干预,探讨AS血管炎性损伤的修复及机制。方法:应用ApoE基因敲除和高脂高胆固醇饲料诱发小鼠AS,分为动脉硬化组、运动组、用药组;ELISA测定血清炎性因子及激素水平;免疫组化法阳性标记TLR4、NF-κb、EGF、TGF-β;RT-PCR测定CRP、MCP-1 mRNA表达。结果:12周动脉硬化组ApoE基因敲除小鼠血管可见明显斑块组织;用药组内皮表层细胞肿胀呈泡沫样,运动组则未见以上组织改变;运动和用药组小鼠血清IL-6、TNF-a含量和血管组织TLR4、NF-κb、CRP、MCP-1表达均降低(P<0.05),而血清IL-10、ET1、AngⅡ和内皮细胞EGF、TGF-βmRNA表达均升高(P<0.05)。结论:有氧运动有效防治AS小鼠血管炎性损伤,其可能机制是介导了Toll样受体通路并恢复血管激素分泌与促进内皮细胞再生。Objective： Indicated of the effects and mechanisms of atherosclerosis vascular inflammation damaged by medium-intensity aerobic exercise and simvastatin pharmaceutical on ApoE knock out mice.Methods： Induced atherosclerosis model through ApoE gene knockout and high fat and high cholesterol fed in mice,and were divided into atherosclerosis groups,exercise groups,drug groups;ELISA assay viewed of serum inflammations and hormone levels;TLR4,NF-κb,EGF,TGF-βlevels detected by immunohistochemistry assay;RT-PCR assay detection of CRP,MCP-1mRNA expression.Results： The atherosclerosis groups showed obvious plaque tissue endothelial cells on ApoE knock out mice for 12 weeks;the drug groups showed endothelial cell swelling and foamy surface,the exercise groups were no more than organizational changed.The serum of IL-6,TNF-a levels and TLR4,NF-κb,CRP,MCP-1expression in vascular of the exercise and drug groups were lower than the quite groups（P0.05）,while IL-10,ET1,AngⅡ levels and EGF,TGF-β mRNA expression increased（P0.05）.Conclusion： It＇s effectively improved vascular inflammation damaged by aerobic exercise,and the possible mechanism mediated Toll-like receptor signaling pathway,while restored hormone secretion and promoted vascular endothelial cell regeneration.

关 键 词：有氧运动 AS 炎性损伤 TLR4通路 

分 类 号：G804.7[文化科学—运动人体科学]