益肾调肝Ⅱ号方对多囊卵巢综合征大鼠卵巢黄体生成素受体表达的影响  

Influence of Yishen Tiaogan Decoction Ⅱ on the Expression of Luteinizing Hormone Receptor of Polycystic Ovarian Syndrome Model Rats

作　　者：陈芊[1] 孙云松[1] 孙选[1] 张斌[1] 徐慧军[1] 

机构地区：[1]青岛市海慈医院妇科,山东省青岛市人民路4号266033

出　　处：《中医杂志》2011年第16期1409-1412,共4页Journal of Traditional Chinese Medicine

基　　金：青岛市2008年度中医科研计划资助项目(青卫中医字2008-ZYW035)

摘　　要：目的观察益肾调肝汤Ⅱ号方对多囊卵巢综合征(PCOS)模型大鼠卵泡发育、卵巢黄体生成素受体(LHR)表达的影响。方法 24日龄Wistar大鼠进行皮下埋植左炔诺孕酮结合皮下注射人绒毛膜促性腺激素造模,将造模成功60只大鼠随机分为6组,每组10只,未造模10只大鼠为正常组。分组灌服生理盐水、盐酸二甲双胍片、益肾调肝汤Ⅰ号方、益肾调肝汤Ⅱ号方低、中、高剂量15天后,观察动物卵巢重量,光镜观察卵巢卵泡的大小、黄体数;免疫组化分析LHR在卵巢中的表达情况。结果运用益肾调肝汤Ⅱ号方中、高剂量后,大鼠发育卵泡数增加,不成熟的早期发育卵泡减少,黄体数量增加,LHR表达增加。结论益肾调肝汤Ⅱ号方能明显增加大鼠卵巢重量、减少小卵泡数、增加黄体数,可明显增强LHR在卵巢中的表达,有利于卵泡的发育及排卵。Objective To observe the intervention effect of Yishen Tiaogan Decoction Ⅱ（Decoction for reinforcing the kidney and regulating the liver） on the follicular development and ovarian luteinizing hormone receptor（LHR） expression of model rats with polycystic ovarian syndrome（PCOS）.Methods Twenty-four-day old female Wistar rats were made PCOS models with embedding levonorgestrel stick subcutaneously in combination with injecting HCG into subcutaneous tissue.Ten rats which were not made models were put into the normal group and the 60 PCOS model rats were randomized into 6 groups,with 10 in each.After intragastric feeding with normal saline,metformin,and Yishen Tiaogan Decoction Ⅰ,low-dose,medium-dose and high-dose of Yishen Tiaogan Decoction Ⅱ respectively for 15 days,the ovary was weighted,the size of follicle and number of luteum were observed with hematoxylin-eosin（HE） staining and optical microscope,and the expression of LHR in ovary was determined with immunohistochemical analysis.Results After using the medium-dose and high-dose of Yishen Tiaogan Decoction Ⅱ,the number of follicles of models rats increased,the immature follicles decreased,the quantity of luteum increased,and the expression of LHR enhanced.Conclusion Yishen Tiaogan Decoction Ⅱ is able to significantly increase the weight of ovary of rats,reduce the number of immature follicles,increase the quantity of luteum,and enhance the LHR expression in ovary,benefiting the development of follicles and ovulation.

关 键 词：益肾调肝汤Ⅱ号方 多囊卵巢综合征 大鼠 黄体生成素受体 

分 类 号：R711.75[医药卫生—妇产科学]