血管生成素及其受体在腹膜透析腹膜组织中的表达  

Expression of angiopoietin and its receptor in peritoneum of peritoneal dialysis rats

作　　者：庄薇[1] 陈红[1] 施敏[2] 钟爱民[1] 

机构地区：[1]江西省人民医院肾内科,南昌330006 [2]江西省肿瘤医院肾内科,南昌330029

出　　处：《临床与实验病理学杂志》2011年第8期852-855,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家自然科学基金卫生部国际合作司项目(30860110)

摘　　要：目的分析并研究尿毒症腹膜透析大鼠腹膜血管生成素(Ang)及其受体(Tie)的表达情况及影响因素。方法 SD雄性大鼠5/6肾切除术建立尿毒症模型,成模后腹腔内植入腹透管,随机分为尿毒症组(NS组)及腹透组(PD组)。尿毒症组每日给予20 ml生理盐水腹腔灌注,腹透组每日给予20 ml 4.25%百特腹透液腹腔灌注,持续6周。对照组(C组)行假手术且不予液体灌注。实验结束取大鼠腹膜组织,观察腹膜血管新生情况;行免疫组化及RT-PCR检测腹膜组织血管生成素及其受体的表达变化。结果实验表明PD组相对其他两组新生血管数明显增多,NS组新生血管数较C组为多(P均<0.05)。免疫组化显示Ang2及其Tie2阳性细胞数PD组相对于C组和NS组明显增加,NS组较C组亦有增加(P均<0.05)。RT-PCR显示PD组Ang2 mRNA和Tie2 mRNA表达较NS组和C组明显上调,而NS组高于C组(P均<0.05)。结论尿毒症本身及腹膜透析液均会上调腹膜的血管生成素及其受体表达,且与血管新生密切相关。Purpose Long-term peritoneal dialysis（PD） can induce ultrafiltration failure,and one of its main factors is angiogenesis.For understanding the cause of peritoneum angiogenesis,the aim of this study was to investigate the change of angiopoietin and its receptor in peritoneum of peritoneal dialysis rats.Methods Uremic rats were established by subtotal nephrectomy in which the rat was implanted a peritoneal dialysis tube.The rats were treated with daily injection of 20 ml physiological saline（NS group） and 20 ml 4.25% peritoneal dialysate（PD groups） by transcatheter,respectively,for 6 weeks.Rats with sham operation without peritoneal dialysate were used as control group.The peritoneum was obtained when the experiment was finished.Vessel density of the peritoneum was detected,and the expression levels of angiopoietin and its receptor were examined in the peritoneum by immunohistochemistry and RT-PCR.Results The number of blood vessels were significantly increased in PD group compared with control group and NS group,and increased in NS group compared with control group（P0.05）.Immunohistochemistry displayed that the positive cells of Ang2 and Tie2 were higher in PD group than that in control and NS groups（P0.05）.The expression levels of Ang2 mRNA and Tie2 mRNA were increased significantly in PD group compared with other two groups,and higher in NS group than that in control group（P0.05）.Conclusion Uremia itself and peritoneal dialysate up-regulate the expression of angiopoietin and its receptor.Angiopoietin and its receptor are closely related with the angiogenesis.

关 键 词：腹膜透析 尿毒症 血管新生 血管生成素 受体 

分 类 号：R692.5[医药卫生—泌尿科学] R364.33[医药卫生—外科学]