罗非鱼维氏气单胞菌的分离鉴定和药敏试验  

Isolation and Identification of Aeromonas veronii from Infected Tilapia Fry and Analysis of Its Drug Sensitivity

作　　者：黎炯[1,2] 叶星[1] 卢迈新[1] 邓国成[1] 迟妍妍[1,2] 黄樟翰[1] 

机构地区：[1]中国水产科学研究院珠江水产研究所,广东广州510380 [2]上海海洋大学水产与生命学院,上海201306

出　　处：《水生态学杂志》2011年第3期132-136,共5页Journal of Hydroecology

基　　金：现代农业产业技术体系建设专项资金资助(No.nycytx-48-4);公益性行业(农业)科研专项(3-49);广东省农业攻关项目(No.2009B020201003);广东省海洋渔业科技推广专项(No.A200901C01)资助

摘　　要：从患病罗非鱼(Oreochromis niloticus)鱼苗的病灶处分离到1株细菌GDZ1,人工感染试验显示,该分离株具有较强毒力。经ATB 32E细菌鉴定系统鉴定、补充生化鉴定和16SrRNA基因序列分析,确定该病原菌为维氏气单胞菌(Aeromonas veronii);药敏实验结果显示,29种药物中,米诺环素等15种化学试剂对该病原菌具有较强的抑菌作用。A pathogenic bacterial strain GDZ1 was isolated from diseased tilapia fry cultured in a farm in Zhuhai,Guangdong.Artificial infection experiments showed that the isolated strain possessed strong virulence.Combining ATB 32E identification,supplementary biochemical identification and 16S rRNA sequence analysis, strain GDZ1 was identified as Aeromonas veronii. The drug sensitivity test of a total of 29 antimicrobial agents showed that the strain GDZ1 was highly sensitive to 15 agents such as minocycline,streptomycin,cefaclor and cefazolin.

关 键 词：罗非鱼 维氏气单胞菌 分离 鉴定 药物 敏感性 

分 类 号：S948[农业科学—水产养殖]