毛细管电泳安培检测法测定密蒙花中的黄酮类化合物  

Determination of Flavonoids in Flos Buddlejae by Capillary Electrophoresis with Amperometric Detection

作　　者：余丽双[1,2] 褚克丹[1] 许雪琴[2] 

机构地区：[1]福建中医药大学药学院,福建福州350108 [2]福州大学食品安全分析与检测教育部重点实验室,福建福州350002

出　　处：《分析科学学报》2011年第4期413-416,共4页Journal of Analytical Science

基　　金：福建省自然科学基金(No.2010J05020);福建省科技重大专项子专题(No.2009YZ0001-1-1)

摘　　要：采用毛细管电泳-安培检测法(CE-AD)对蒙花苷、刺槐素、木犀草素和芹黄素四种黄酮类化合物进行分离分析。在电极电位为+0.95V(vs.Ag/AgCl),电泳运行液为pH=9.00的60mmol/L Na2B4O7-120mmol/L NaH2PO4缓冲溶液,分离电压为18kV时,四种黄酮类化合物得到完全分离。在最优的条件下,四种黄酮类化合物的检出限(S/N=3)为0.2～0.005μg/mL。该方法可用于中药密蒙花中上述黄酮类化合物含量的测定。A method based on capillary electrophoresis with amperometric detection（CE-AD） was developed for identification and determination of four flavonoids（linarin,acacetin,apigenin and luteolin） reported in plant species.Under the optimum conditions（detection potential of ＋0.95 V（vs.Ag/AgCl）,running buffer of 60 mmol/L Na2B4O7-120 mmol/L NaH2PO4（pH=9.00） and separating voltage of 18 kV）,the four flavonoids were well separated.Optimized detection provided detection limit（defined as S/N=3） ranging from 0.2 μg/mL to 0.005 μg/mL for all analytes.The proposed method was applied for the determination of the four flavonoids in Flos buddlejae,and the assay results were satisfactory.

关 键 词：黄酮类化合物 密蒙花 毛细管电泳 安培检测 

分 类 号：O657.8[理学—分析化学]