塑料玩具中丙烯酰胺总量及迁移量的高效液相色谱检测法及确证  

Determination of Acrylamide and Its Migration in Plastic Toys by High Performance Liquid Chromatography

作　　者：王烨[1] 马强[1] 白桦[1] 王超[1] 席海为[1] 刘茜[1] 

机构地区：[1]中国检验检疫科学研究院,北京100123

出　　处：《分析科学学报》2011年第4期435-438,共4页Journal of Analytical Science

基　　金：质检公益性行业科研专项计划(No.200810953);国家质量监督检验检疫总局科技计划项目(No.2010IK088);国家认证认可监督管理委员会计划(No.2007B259)

摘　　要：建立了一种简单、快速测定玩具中丙烯酰胺含量与迁移量的高效液相色谱法(HPLC)。该方法以Waters Atlantis T3柱为分析柱,以纯水超声提取并作为流动相,采用HPLC对水中丙烯酰胺的含量及模拟汗液、唾液中丙烯酰胺的迁移量进行测定,并测定了自制阳性样品中的残留丙烯酰胺的含量,最后用液相色谱-串联质谱(LC-MS/MS)进行确证。丙烯酰胺的回收率在97.49%～103.42%之间,相对标准偏差(RSD)为0.66%～4.24%,检出限为0.02mg/L。An easy and fast high performance liquid chromatographic（HPLC） method has been developed for the determination and quantification of acrylamide in plastic toys.This method was performed on a Waters Atlantis T3 column with pure water as mobile phase.Acrylamide was extracted by ultrasonator,detected by HPLC and verified by LC-MS/MS.The concentrations of acrylamide in self-made positive sample and the migration of acrylamide in simulated sweat and saliva were determined.The recoveries of acrylamide were among 97.49%～103.42%,with the relative standard deviations among 0.66%～4.24%,and the limit of detection was 0.02 mg/L.

关 键 词：丙烯酰胺 塑料玩具 高效液相色谱 自制阳性样品 迁移量 

分 类 号：O657.72[理学—分析化学]