荧光光谱法研究染料木苷与牛血清白蛋白的相互作用  

Study on the Interaction of Genistin with Bovine Serum Albumin by Fluorescence Spectroscopy

作　　者：宋胜梅[1,2] 汤文芳 郭晓莉[2] 屈凌波[1] 徐茂田[2] 

机构地区：[1]郑州大学化学系,河南郑州450001 [2]商丘师范学院化学系,河南省高校重点实验室培育基地,河南商丘476000 [3]河南省农业科学研究院畜牧兽医研究所,河南郑州450001

出　　处：《分析科学学报》2011年第4期459-462,共4页Journal of Analytical Science

基　　金：国家自然科学基金(No.20775047)

摘　　要：采用荧光光谱法,在pH=7.4的磷酸盐缓冲溶液(PBS)中研究了染料木苷(Genistin,Ge)与牛血清白蛋白(BSA)的作用方式及作用机理。结果表明,Ge可静态猝灭BSA的内源荧光,二者之间的结合位点数为1.4,温度对结合常数影响不大,300K、306K、310K和313K的结合常数平均为4.31×104 L.mol-1。热力学参数表明二者之间的作用力主要是静电力。同步荧光光谱表明Ge使色氨酸残基的微环境亲水性增强。本研究对于阐明Ge与蛋白的相互作用机理具有借鉴意义。The interaction mechanism and mode of genistin（Ge） with bovine serum albumin（BSA） in pH 7.4 PBS were studied by fluorescence spectroscopy.The results showed that the intrinsic fluorescence of BSA could be quenched by Ge.Furthermore,the number of binding site of 1.4 was obtained.And the average apparent binding constant at 300 K,306 K,310 K and 313 K was 4.31×104 L／5mol-1.The thermodynamic parameters determined by van＇t Hoff analysis indicated that the type of force was electrostatic force.Synchronous fluorescence spectra showed that the microenvironment of tryptophan residues was more hydrophilic when Ge was added.So,this study could be significant for understanding the interaction mechanism between Ge and proteins.

关 键 词：染料木苷 牛血清白蛋白 荧光光谱 

分 类 号：O657.39[理学—分析化学]