威灵仙皂苷对急性早幼粒细胞白血病细胞株NB_4细胞的凋亡诱导作用及其机制  

Radix Clematidis Saponins Inhibits NB_4 Cells and through Inducement of Apoptosis

作　　者：周云[1] 黄纯兰[2] 李录克[1] 李晓明[2] 

机构地区：[1]凉山州第一人民医院血液科,四川西昌615000 [2]泸州医学院附属医院血液科

出　　处：《肿瘤防治研究》2011年第8期881-885,共5页Cancer Research on Prevention and Treatment

基　　金：四川省卫生厅基金资助项目(07023);泸州市科技局基金资助项目(07073);泸州医学院附属医院人才基金资助项目(07043)

摘　　要：目的探讨威灵仙皂苷对急性早幼粒细胞白血病细胞株NB4细胞的凋亡诱导作用及其机制。方法以NB4细胞为实验对象,1.0μmol/L的三氧化二砷为阳性对照,0.01%DMSO为阴性对照,用不同浓度的威灵仙皂苷作为实验组,利用MTT法观察细胞的增殖抑制状态,瑞氏染色进行细胞形态学观察,流式细胞术检测细胞凋亡率及进行细胞周期测定,Real-time PCR检测威灵仙皂苷干预细胞后PML-RARa mRNA的变化。结果不同浓度的威灵仙皂苷作用细胞后,均能抑制细胞增殖,具有时间和浓度依赖性,但抑制率低于砷剂组。瑞氏染色可见典型凋亡细胞的形态学改变,与砷剂组有同样的改变。流式细胞术检测发现凋亡细胞随时间延长而增多,组间差异有统计学意义,凋亡率低于砷剂组。细胞周期测定提示G2期细胞增多,G1期细胞明显减少,组间差异有统计学意义(P<0.05)。威灵仙皂苷作用后的NB4细胞PML-RARa mRNA的表达无统计学意义(P>0.05)。结论威灵仙皂苷可能通过诱导NB4细胞凋亡抑制细胞生长,可能的机制是通过细胞周期阻滞,但不影响PML-RARa mRNA的表达。Objective To investigate the effect of Radix Clematidis Saponins（RCS） on NB4 cells apoptosis and its possible mechanism.Methods NB4 cells were treated with different concentrations of Radix Clematidis Saponins,using 0.01% DMSO as the negative control group,1.0 μmol/L Arsenic Trioxide as the positive control group.MTT method was used to detect the inhibition rate.The apoptostic rate and the alteration of cell cycles were assessed by flow cytometry determination and cell morphology was observed by wright＇s staining.Real-time PCR was used to evaluate the effects of sRadix Clematidis Saponins on the PML-RARa mRNA.Results Radix Clematidis Saponins was able to inhabit the NB4 cell growth,in time or concentration-dependent manner,but the inhibition rate was less than Arsenic Trioxide group.Wright＇s staining showed typical apoptotic morphological changes in Radix Clematidis Saponins group,the same as Arsenic Trioxide group.Apoptotic rate was increased in time dependent manner.The number of G2 stage cells was increased,while G1 stage was decreased（P0.05）.There was no difference of expression of PML-RARa mRNA between two groups or between different time periods of Radix Clematidis Saponins treatment.Conclusion Radix Clematidis Saponins might induce cell apoptosis,possibly by cell cycle arresting,but not reduce the PML-RARa mRNA gene expression.

关 键 词：威灵仙皂苷 急性早幼粒细胞性白血病 NB4细胞 细胞凋亡 

分 类 号：R932[医药卫生—生药学] R733.71[医药卫生—药学]