青蒿琥酯对人宫颈癌细胞放射增敏作用的影响研究  

The study on the effect of artesunate on the radio-sensitivity of human cervical cancer

作　　者：耿冲[1] 曹建平[1] 倪倩影[1] 朱巍[1] 张旭光[2] 陈秋[1] 杨巍[1] 张舒羽[1] 封阳[1] 周媛媛[1] 罗居东[1] 陈光烈[1] 

机构地区：[1]苏州大学放射医学与公共卫生学院,苏州215123 [2]徐州市肿瘤医院,徐州221100

出　　处：《辐射研究与辐射工艺学报》2011年第4期238-242,共5页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金(30670632);"长江学者和创新团队发展计划资助"(IRT0849)资助

摘　　要：本工作主要涉及青蒿琥酯对体外培养人宫颈癌HeLa、Siha细胞株的放射增敏作用。实验选择人宫颈癌细胞HeLa、Siha作为实验细胞。利用四氮唑盐比色试验(MTT)确定细胞辐射损伤实验最佳药物浓度;常规染色体畸变法、胞质分裂阻滞微核法(CB法)分别研究青蒿琥酯对辐射诱发人宫颈癌细胞染色体畸变、微核的影响。确定了青蒿琥酯的实验药物浓度:HeLa为2.0μg/mL、Siha为4.0μg/mL。结果表明,HeLa细胞经青蒿琥酯作用并在相同剂量辐照后,染色体畸变率、微核细胞率及微核率较单纯照射增加明显;Siha细胞在相同辐照剂量下加药处理组与单纯照射组比较,染色体畸变率、微核细胞率及微核率均无统计学差异(p>0.05)。To investigate the effect of artesunate on radio-sensitivity of human cervical cancer cells in vitro, The human cervical cancer cells HeLa and Siha were used as the experimental cells. MTT assay was used to determine the most appropriate drug concentration in the subsequent experiment, and the effect of human cervical cancer cells treated with artesunate and irradiation of 6~Co ~/-rays was studied by using conventional chromosomal aberration analysis and cytokinesis block method （CB method）. The results show that when the concentration of artesunate in this experiment was 2.0 μmol/mL for HeLa cell and 4.0 μmol/mL for Siha cell respectively, the chromosome aberration, micronuclei cell and micronuclei rates of HeLa cells treated with artesunate were more serious than that of the only irradiation, but there is almost no change with Siha cells.

关 键 词：青蒿琥酯 人宫颈癌细胞 放射增敏 染色体畸变法 微核法 

分 类 号：R811.5[医药卫生—放射医学] R394[医药卫生—临床医学]