电离辐射和紫杉醇诱导的KB细胞中SPATA5L1和Cyclin B2表达的变化  

The expression of the SPATA5L1 and Cyclin B2 in KB cell induced by irradiation and paclitaxel

作　　者：杨丹[1] 许玉杰[1] 白洁[1] 翁婉雯[1] 丁文秀[1] 洪承皎 

机构地区：[1]苏州大学医学部放射医学与公共卫生学院基础核医学教研室,苏州215123 [2]江苏省放射医学与防护重点实验室,苏州215123

出　　处：《辐射研究与辐射工艺学报》2011年第4期243-248,共6页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金(30970873);江苏省高校自然科学基金(04KJB350130);江苏省放射医学与防护重点实验室开放课题(KJS05030)资助

摘　　要：电离辐射与紫杉醇协同作用,可以诱导体外培养的人口腔上皮癌KB细胞出现G2/M阻滞,形成多核细胞,并最终造成死亡。先前通过基因芯片筛选研究发现,在经过电离辐射和/或紫杉醇作用后,有10条与细胞分裂有关的基因存在差异表达。本研究旨在通过Real-time PCR和Western Blot定量分析其中两条基因SPATA5L1和CyclinB2的表达变化,来揭示G2/M阻滞及多核细胞形成的分子机制。结果表明,在受到6 Gy以下不同剂量的电离辐射或电离辐射与40 nmol/L紫杉醇协同作用后,SPATA5L1的表达量明显增加,但同时Cyclin B2的表达量则下降。推测SPATA5L1上调表达和CyclinB2适度的下调表达,使受处理细胞在胞核分裂后阻止胞浆分裂而无法形成正常子代细胞,导致G2/M阻滞及多核细胞形成。The G2/M arrest of human oral epithelial carcinoma KB cells could be induced by the synergistic effect of ionizing radiation with paclitaxel, which results in the formation of multinucleated giant cells and eventually cell death. Earlier biochip screening analysis found that ten genes related to regulate cell division have been differential expression after treated with ionizing radiation and/or paclitaxel. This study was to investigate the potential molecular mechanism by quantitative assessment two gene expression levels among the ten genes. The SPATA5L1 gene was quantified by real-time quantitative PCR. And the protein expression levels of Cyclin B2 and SPATA5L1 were detected by Western Blot. The results show that the expression of SPATA5L1 gene increase after different doses irradiation （below 6 Gy） or irradiation combined with 40 nmol/L paclitaxel, however, the expression of Cyclin B2 de- crease at the same time. We suppose that the expression of up-regulated SPATA5L1 and down-regulated Cyclin B2 maybe play a crucial role in the G2/M arrest and the formation of multinucleated giant cells, which block the cytoplasm split to form new daughter cell after nucleolus division.

关 键 词：紫杉醇 KB细胞 多核细胞 SPATA5L1 CyclinB2 

分 类 号：R811.5[医药卫生—放射医学] R815.5[医药卫生—临床医学]